The stimulation in vitro of sodium transport in the toad bladder by aldosterone and other mineralocorticoids is well established (l-3). Sodium transport is stimulated by physiological concentrations of aldosterone, and the effect of aldosterone is inhibited by spirolactones, progesterone, and ouabain (4). Evidence has been obtained which suggests the involvement of protein synthesis in the mech...

NEELY, ,J. R., H. LIEBERMEISTER. E.,J. BATTERSBY, AND H. E. MORGAN. fi;fect of pressure development on oxygen consumption by isolcrted rot heart. Am. J. Physiol. 2 I 2 (4) : 804-814. I g67.-The isolated perfused rat heart has been modified to allow metabolic studies over a range of heart work and pressure development. When the left atria1 filling pressure was increased from o to 20 cm H20, card...

PURPOSE The Yukon Arctic Ultra is considered the longest and coldest ultraendurance event in the world. Cold exposure and exercise has been reported to influence circulating levels of myokines, adipokines, and hepatokines that may influence considerable alterations in the regulation of metabolism. The purpose of the study was to evaluate the influence of the Yukon Arctic Ultra (430-mile event) ...

The occurrence of imine formation at the active site of acetoacetate decarboxylase and several aldolases prompted an attempt to demonstrate this mechanism in the formally similar isocitrate dehydrogenase reaction. Although treatment of isocitrate dehydrogenase with NaBH4 in the presence of cr-ketoglutarate resulted in inactivation of the enzyme, reduction in the presence of labeled a-ketoglutar...

From Table 2 it will also be seen that the diabetogenic dose of alloxan in normal animals, as found in this laboratory, ranges between 130 and 140 mg./kg. and the lethal dose is observed to be in the neighbourhood of 225 mg./kg. It is interesting to note that the diabetogenic and lethal doses of alloxan in acetoacetate-treated animals, having blood GSH in the neighbourhood of 3 mg./100 ml., are...

Several amino acids were found to stimulate glycogen synthesis and lipogenesis, and to inhibit ketogenesis in isolated rat hepatocytes. When hepatocytes were incubated in the presence of 20 mM-glucose, the amino acids could be classified in decreasing order of efficiency as follows: glutamine and proline, alanine, aminoisobutyric acid, asparagine and histidine for stimulation of glycogen synthe...

The amounts of succinyl-CoA-3-oxo acid CoA-transferase (EC 2.8.3.5) decrease progressively in skeletal muscle in streptozotocin-diabetic rats, reaching after 10 days about 50% of the value in normal rat muscle. Electrofocusing studies indicate the occurrence of partial proteolysis of the enzyme in diabetic muscle. However, several functional parameters relating to acetoacetate utilization, incl...

Arteriovenous glucose difference across the mammary gland of the lactating rat was used as an 'instantaneous' monitor of mammary glucose uptake. Plasma [glucose] and arteriovenous glucose difference varied according to whether Halothane, diethyl ether or sodium pentobarbitone anaesthesia was used. In pentobarbitone-treated rats a 60% glucose extraction in the fed state decreased to 5% after 18 ...

An enzymatic kit method for the determination of plasma creatinine was optimised for use with a centrifugal analyser and its performance characteristics and practicability compared with an end point and a kinetic Jaffé-based method. The enzymatic method exhibited several advantages over Jaffé-based methods--namely, smaller sample size, rapid sample throughput (200 per hour), and improved specif...

Concentrations of acetoacetate and P-hydroxybutyrate and their specific radioactivities were shown to be assayable directly in plasma of the rat without the need for prior deproteinization. By utilizing this technique ketone body turnover was estimated in the isotopic steady state and after the single injection of radioactive acetoacetate or P-hydroxybutyrate. While difEculties exist in both ty...