Journal:
:jundishapur journal of microbiology
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mohammad hossein morowvat department of pharmaceutical biotechnology, faculty of pharmacy, shahid beheshti university of medical sciences, tehran, ir iran; pharmaceutical sciences research center, shiraz university of medical sciences, shiraz, ir iran
valiollah babaeipour biochemical engineering group, biotechnology research center, malek-ashtar university of technology, tehran, ir iran; department of bioscience engineering, faculty of new sciences and technologies, university of tehran, tehran, ir iran; department of bioscience engineering, faculty of new sciences and technologies, university of tehran, p. o. box 14395-1374, tehran, ir iran. tel/fax: +98-2122974614
hamid rajabi memari department of agronomy and plant breeding, college of agriculture, shahid chamran university of ahvaz, ahvaz, ir iran
hossein vahidi department of pharmaceutical biotechnology, faculty of pharmacy, shahid beheshti university of medical sciences, tehran, ir iran
conclusions it can be concluded that the rsm is an effective method for the optimization of recombinant protein expression using synthetic genes in e. coli. results the proposed optimum fermentation condition consisted of 7.81 g l-1 glucose, od 600 nm prior induction 1.66 and induction temperature of 30.27°c. the model prediction of 0.267 g l-1 of rhifn-β and 0.961 g l-1 of acetate at the optim...