Knowledge about the cytology and reproductive behavior of a species is indispensable for hybridization programs. This is especially true for species belonging to the genus Paspalum, among which apomixis and a wide range of ploidy levels are frequently found. Paspalum conspersum Schrad. is a robust and warm-season perennial bunchgrass native to South America. Previous studies have indicated that...

Bacterial wilt caused by Ralstonia solanacearum is a disease of widespread economic importance that affects numerous plant species, including Arabidopsis thaliana. We describe a pathosystem between A. thaliana and biovar 3 phylotype I strain BCCF402 of R. solanacearum isolated from Eucalyptus trees. A. thaliana accession Be-0 was susceptible and accession Kil-0 was tolerant. Kil-0 exhibited no ...

Next-generation sequencing technologies are accelerating gene discovery by combining multiple steps of mapping and cloning used in the traditional map-based approach into one step using DNA sequence polymorphisms existing between two different accessions/strains/backgrounds of the same species. The existing next-generation sequencing method, like the traditional one, requires the use of a segre...

Sequence tagged site (STS) markers have been developed recently to identify resistance genes in wheat. A number of wild relatives have been used to transfer resistance genes into wheat cultivars. Accessions of wild species of Triticeae: Aegilops longissima (4), Ae. speltoides (6), Ae. tauschii (8), Ae. umbellulata (3), Ae. ventricosa (3), Triticum spelta (2), T. timopheevi (3), T. boeoticum (4)...

nucleotide substitutions in position 198 (T→C) and in position 136 (A→G) of the 249-bp fragment. The remaining sample showed 100% identity with B. henselae strains Brazil-1 and 4500249 (GenBank accession nos. HQ012580 and GQ225709). Four of the 56 successfully sequenced samples (7.14%; all samples from nymphs) showed the sequence pattern of B. grahamii. One sample revealed 100% identity with B....

OBJECTIVE To evaluate the association of pre-accession physical fitness, as measured by a five-minute step test, with incidence of overuse injuries and outpatient healthcare utilization among male United States (US) Army recruits. PARTICIPANTS US Army male trainees who met weight standards and took a pre-accession fitness test. METHODS In this prospective cohort study, incidence and outpati...

The phylogenetic analysis of nucleotide sequences and increasingly that of amino acid sequences is used to address a number of biological questions. Access to extensive datasets, including numerous genome projects, means that standard phylogenetic analyses can include many hundreds of sequences. Unfortunately, most phylogenetic analysis programs do not tolerate the sequence naming conventions o...