Heat stress (HS) has become one of the major abiotic stresses that severely constrain rice growth. Abscisic acid (ABA) plays an important role in plant development and response. However, effect different concentrations exogenous ABA on HS tolerance still needs to be further elucidated. Here, we found high increased damage seedlings, whereas 10−12 M treatment fresh dry weight under relative mock...

Abstract Abscisic acid (ABA) signaling is critical for seed germination and abiotic stress responses in terrestrial plants. Pre-mRNA splicing known to regulate ABA signaling. However, the involvement of canonical spliceosomal components regulating poorly understood. Here, we show that spliceosome component Sm core protein SmEb plays an important role expression up-regulated by treatment, analys...

Abscisic acid (ABA) is a major phytohormone that regulates abiotic stress responses and development. SNF1-rerated protein kinase 2 (SnRK2) key regulator of ABA signaling. To isolate compounds which directly affect SnRK2 activity, we optimized fluorescence-based system for high-throughput screening (HTS) regulators. Using this system, screened chemical library consisting 16,000 identified ten (I...

Abstract Although it is well established that nitrogen (N) deficiency induces leaf senescence, the molecular mechanism of N deficiency-induced senescence remains largely unknown. Here, we show an abscisic acid (ABA)-responsive NAC transcription factor (TF) involved in senescence. The overexpression MdNAC4 led to increased ABA levels apple calli by directly activating biosynthesis gene MdNCED2 ....

While abscisic acid (ABA) exerts multiple effects on somatic embryogenesis, the most pronounced of these effects is the arrestment of torpedo-stage embryos, preventing them from developing into plantlets. In order to understand the mechanism of ABA inhibition of plantlet formation, we have isolated seven ABA-insensitive cell lines capable of developing into plantlets in the presence of ABA. The...

Nitric oxide (⋅NO) is known to attenuate dormancy and promote germination, a function that seemingly depends on crosstalk with the abscisic acid (ABA) signaling network. In the past 2 years, a number of independent studies have revealed that ⋅NO gates the ABA signaling network at multiple steps, ensuring redundant and effectively irreversible control of germination. Here we summarize the recent...

The phytohormone abscisic acid (ABA) plays a key role in the plant response to drought stress. Hence, ABA-dependent gene transcription and ion transport is regulated by a variety of protein kinases and phosphatases. However, the nature of the membrane-delimited ABA signal transduction steps remains largely unknown. To gain insight into plasma membrane-bound ABA signaling, we identified sterol-d...

Two rice varieties N22 (drought-tolerant) and Panidhan (drought-susceptible) were studied for the protein profiles under control, moisture stress and abscisic acid (ABA) treatments. Both qualitative and quantitative differences were observed. In control and ABA treatment, the number of polypeptides was more in N22, compared to Panidhan. However, a significant number of polypeptides were induced...

Abscisic acid (ABA) is an important plant hormone that modulates seed germination and plant growth and stress responses, but its signaling remains poorly understood. We investigated the role of ROP10, a member of the Arabidopsis Rop subfamily of Rho GTPases, in ABA signaling. A null rop10 mutant exhibits enhanced responses to ABA in seed germination, root elongation, and stomatal closure assays...

An abscisic acid (ABA)-insensitive Vicia faba mutant, fia (fava bean impaired in ABA-induced stomatal closure) had previously been isolated. In this study, it was investigated how FIA functions in ABA signalling in guard cells of Vicia faba. Unlike ABA, methyl jasmonate (MeJA), H(2)O(2), and nitric oxide (NO) induced stomatal closure in the fia mutant. ABA did not induce production of either re...