The contact system comprises a series of serine proteases that mediate procoagulant and proinflammatory activities via the intrinsic pathway coagulation kallikrein–kinin system, respectively. Inhibition Factor XIIa (FXIIa), an initiator has been demonstrated to lead thrombo-protection anti-inflammatory effects in animal models serves as potentially safer target for development antithrombotics. ...

An important objective of computational protein design is the generation of high affinity peptide inhibitors of protein-peptide interactions, both as a precursor to the development of therapeutics aimed at disrupting disease causing complexes, and as a tool to aid investigators in understanding the role of specific complexes in the cell. We have developed a computational approach to increase th...

Objectives: Capping protein Arp2/3 and myosin-I linker 1 (CARMIL1) encoded by the CARMIL, is a major, multidomain, membrane-linked regulating actin assembly; however, its function in inflammatory signaling not fully elucidated. The leucine-rich repeat (LRR) region of CARMIL1 has been associated with interleukin (IL)-1 receptor-associated kinase (IRAK) fibroblasts many methods including tandem m...

Among gene delivery systems, peptide-based carriers have received significant attention because of their selectivity, biocompatibility, and biodegradability. Since cellular membranes function as a barrier toward exogenous molecules, cell-penetrating peptides (CPPs), which are usually cationic and/or amphiphilic, can serve efficient to deliver cargo into the cytosol. Here, we examined interactio...

The physical association of 40 antigenic peptides and purified HLA class I and class II molecules was monitored using a direct peptide binding assay (PBA) in solid phase and an inhibition peptide binding assay (IPBA) in which the competing peptide was present in a soluble phase. We also examined the ability of different peptides to inhibit the lytic activity of human antiviral cytolytic T cells...

Arginine-rich peptides and small-molecule intercalating agents utilize distinct molecular mechanisms for RNA recognition. Here, we combined these distinct binding modules in an effort to create conjugate ligands with enhanced affinity and specificity using the bacteriophage lambda N peptide-boxB interaction as a model system. We first designed and synthesized a series of peptide-acridine conjug...

Novel antihypercholesterolemic bioactive peptides (BAP) from peptic camel whey protein hydrolysates (CWPH) were generated at different time, temperature, and enzyme concentration (%). Hydrolysates showed higher pancreatic lipase- (PL; except 3 CWPH) cholesterol esterase (CE)-inhibiting potential, as depicted by lower half-maximal inhibitory values (IC50 values) compared with nonhydrolyzed prote...

We analyzed the binding of an influenza matrix protein-derived peptide, MAT(17-31), to cell surface and purified DR1. The pH dependence of peptide binding was dramatically influenced by the membrane environment. Cell surface binding was enhanced at low pH, with little or no binding detected at neutral pH and optimal binding at pH 4. By contrast, hydrogen ion concentration had minimal effect on ...

UNLABELLED Peptides are useful tools for directing radioisotopes into tumors. We evaluated the ability of a bacterial peptide display system to isolate new prostate tumor-specific peptides. METHODS We used the bacterial FliTrx system to identify a new cyclic peptide that binds to prostate carcinoma. Serum stability and binding affinities of the (125)I-labeled peptide were tested. Furthermore,...

We analyzed the binding of an influenza matrix protein-derived peptide, MAT(17-31), to cell surface and purified DR1. The pH dependence of peptide binding was dramatically influenced by the membrane environment. Cell surface binding was enhanced at low pH, with little or no binding detected at neutral pH and optimal binding at pH 4. By contrast, hydrogen ion concentration had minimal effect on ...