نتایج جستجو برای: a 355 bp fragment was amplified from s
تعداد نتایج: 14809709 فیلتر نتایج به سال:
there are two major theories of measurement in psychometrics: classical test theory (ctt) and item-response theory (irt). despite its widespread and long use, ctt has a number of shortcomings, which make it problematic to be used for practical and theoretical purposes. irt tries to solve these shortcomings, and provide better and more dependable answers. one of the applications of irt is the as...
conclusions all the bacterial isolates were associated with a high resistance to various antibiotics and this resistant pattern could be confirmed by detection of a particular gene for each bacterium. objectives this study examined bacteremia in cancer patients from two medical centers regarding isolates and spectrum of antibiotic resistance pattern. patients and methods this was a prospective ...
A molecular maker for authenticating species origin of the stingless bee (Trigona collina) was developed. Initially, amplified fragment length polymorphism analysis was made of 11 stingless bee species using 64 primer combinations. A 316-bp band found only in T. collina was cloned and sequenced. A primer pair (CUTc1-F/R) was designed and tested for species-specificity in 15 stingless bee specie...
In order to distinguish Korean cattle (Hanwoo) beef from the imported beef from Australia in Korean markets, DNA markers based on PCR-RFLP from mitochondrial genes and SRY gene were applied. A total of 2,826 beef samples comprising 1,495 Hanwoo and 1,331 foreign cattle breeds were obtained in Korea. An 801 bp fragment of the SRY gene on the bovine Y chromosome, a 343 bp fragment of ND4 gene and...
worship is submission and humitility with believe to divinity in fornt of god. in jewish religion ,worship is submission of the god inall the ordes and devosion tohim .worshio is not only so widely in islam and jew and does not include pray and fast and etc but also is every action that human does with divine intention and to content him. worship has huge and excellent rank in qoran and old te...
A method for high-resolution genomic fingerprinting of the enteric pathogens Campylobacter jejuni and Campylobacter coli, based on the determination of amplified fragment length polymorphism, is described. The potential of this method for molecular epidemiological studies of these species is evaluated with 50 type, reference, and well-characterised field strains. Amplified fragment length polym...
In three-line system, cytoplasmic male sterile (CMS) lines often were contaminated with cognate iso-nuclear maintainer lines during seeds multiplication processes. Therefore fingerprinting of breeding lines and identification of line-specific markers are prerequisite in genetic purity test. Six CMS lines including Neda-A, Nemat-A, Dasht-A, Amol 3-A, Champa-A, IR58025A and their iso-nuclear main...
Prolactin is secreted from the anterior pituitary gland and plays an important role in the mammary gland development, differentiation and lactation. The aim of current study was to evaluate prolactin receptor gene polymorphisms in the Shal breed and Shal×Romanov crossbreeds. For this purpose, after blood sampling of animals and extraction of DNA using phenol chloroform method, two fragments of ...
A PCR assay, using three primer pairs, was developed for the detection of Ureaplasma urealyticum, parvo biovar, mba types 1, 3, and 6, in cultured clinical specimens. The primer pairs were designed by using the polymorphic base positions within a 310- to 311-bp fragment of the 5' end and upstream control region of the mba gene. The specificity of the assay was confirmed with reference serovars ...
The claR of Streptomyces clavuligerus in the clavulanic acid gene cluster encodes a transcriptional regulator that controls clavulanic acid biosynthesis. The main goal of this study was isolation and molecular detection of the claR gene and its cloning in the Streptomyces specific vector (pMA:: hyg). By cinsideration of the claR gene’s start codon, the specific primers were designed. After geno...
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