Immortal cell lines have not yet been reported from Penaeus monodon, which delimits the prospects of investigating the associated viral pathogens especially white spot syndrome virus (WSSV). In this context, a method of developing primary hemocyte culture from this crustacean has been standardized by employing modified double strength Leibovitz-15 (L-15) growth medium supplemented with 2% gluco...

Immediate culture or refrigeration of urine is recommended, but not always practical. Therefore, we evaluated the Becton-Dickinson Urine Culture Kit containing a boric acid-glycerol-sodium formate preservative in a study of 1,000 clinical urine specimens. Each specimen was cultured a total of four times by the surface streak technique with a 0.001-ml calibrated loop. After an initial reference ...

We studied the effect of fibrinogen degradation products D, E, and D-dimer on a human promonocytic leukemia cell line, NOMO-1. After exposure to a 10(-5)-mol/L fragment D or D-dimer, the cells displayed macrophage-like characteristics, such as adherence to plastic surfaces, and showed approximately a twofold increase in response to the nitroblue tetrazolium reduction test. The secretion of inte...

OBJECTIVE An efficient anaerobic culture system, the InPouch TV test, was used to determine the susceptibility of Trichomonas vaginalis to metronidazole. Glacial acetic acid was employed as a solvent for metronidazole. METHODS T vaginalis isolates were cultured from 16 symptomatic female patients. The 11 who responded to oral metronidazole, 250 mg tid for 7 days, were considered as having sen...

Trypanosoma danilewskyi strain F Cc-1 was isolated from the blood of a laboratory infected juvenile common carp. Trypanosomes (blood stream forms) were first adapted to culture conditions in Tobie’s medium. Ten sterilized glass culture tubes each containing 2 ml of Tobie’s medium solidified in slants and covered with 1 ml Locke-Krebs overlay, were inoculated with 0.1 ml of infected blood contai...

(Fig. 1).3) Butirosin (Fig. 2) has the L-HABA structure as an acylated side chain on its 2-deoxystreptamine molecule. The structural analogies between AMK and BTR suggest that AMK could be formed by feeding KMA to a broth culture of B. circulars. Recent reports on the biosynthesis of BTR4•5) indicate that the coupling of L-HABA to the ribostamycin (RBT) molecule (Fig. 2) could be one of the las...

The objective of this study was to determine if the presence of porcine growth hormone (pGH) during porcine oocyte in vitro maturation (IVM) affected subsequent embryo development. Pig cumulus-oocyte complexes (COC) (n=987) were aspirated from slaughterhouse derived ovaries and cultured in BSA-free NCSU-23 medium containing porcine follicular fluid (10% v/v), cysteine (0.1 mg/ml) and hormonal s...

Cell culture Cell culture techniques have been described previously. C1 hiPSCs and the hESC lines WIBR#3 were maintained on mitomycin C inactivated mouse embryonic fibroblast (MEF) feeder layers in hESC medium [DMEM/F12 (Invitrogen) supplemented with 15% fetal bovine serum (FBS) (Hyclone), 5% KnockOutTM Serum Replacement (Invitrogen), 1 mM glutamine (Invitrogen), 1% nonessential amino acids (In...

The gastrointestinal tract of bovines often contains bacteria that contribute to disorders of the rumen, and may also contain foodborne or opportunistic human pathogens as well as bacteria capable of causing mastitis in cows. Thus there is a need to develop broad-spectrum therapies that are effective while not leading to unacceptably long antibiotic withdrawal times. The effects of the CH(4)-in...