We previously identified the carbohydrate determinant sialyl 6-sulfo Lewis X (Le(x)) as the major L-selectin ligand on high endothelial venules of peripheral lymph nodes. In this study, we examined the distribution of the sialyl 6-sulfo Le(x) determinant among peripheral lymphocytes. The determinant was expressed on a subset of helper memory T and NK cells. The helper memory T cells expressing ...

We used enzyme (acid phosphatase [AP]) and immunohistochemical techniques and a set of monoclonal antibodies (CD11, CD5, CD4, CD19, CD8, OKIa), including two recently developed antibodies--for example, HECA-452 (specific for an adhesion molecule on high endothelial venules) and RFD1 (specific for 'active' human dendritic cells) to analyse the composition of the gut wall infiltrate of 10 well de...

Hieroglyph Demotic Greek From 452 to 1822, "Egypt was silent": The ability to read hieroglyphic inscriptions on monuments and tombs, and papyrus texts using cursive scripts like demotic, was lost.

For detection Aleutian mink disease virus (AMDV) antibodies, an ELISA was 26 developed using the recombinant VP2332-452 protein as an antigen. Counter 27 immunoelectrophoresis (CIEP) was used as a reference test to compare the results of enzyme 28 linked immunosorbent assay (ELISA) and western blotting (WB), the specificity and sensitivity of 29 VP2332-452-ELISA was 97.9% and 97.3%, respectivel...

MicroRNAs (miRNAs), a group of small non-coding RNAs, can regulate gene expression by triggering translation repressing and/or RNA degradation. Recent studies proved that they were deeply involved in tumorigenesis. Here we show that, compared with the two normal prostate cell lines, eighteen miRNAs in the array were over-expressed and 18 showed lower levels of expression in at least 2 of 3 canc...

L-selectin mediates leukocyte rolling on vascular endothelium during inflammation. Although vascular endothelium can be activated with inflammatory cytokines to express functional L-selectin ligands, these ligands have not been well characterized. In this study, fucosyltransferase VII cDNA (Fuc-TVII) transfection of the EA.hy926 human vascular endothelial cell line (926-FtVII) induced functiona...

BACKGROUND L-selectin ligands, localized to the luminal epithelium at the time of implantation, may support the early stages of blastocyst attachment. We have assessed the expression of two L-selectin ligands, defined by MECA-79 and HECA-452 monoclonal antibodies, and the sulfotransferase GlcNAc6ST-2, involved in generation of L-selectin ligand epitopes, in the secretory phase of the endometriu...

Neural crest cells (NCCs) are a subset of multipotent, migratory stem cells that populate a large number of tissues during development and are important for craniofacial and cardiac morphogenesis. Although microRNAs (miRNAs) have emerged as important regulators of development and disease, little is known about their role in NCC development. Here, we show that loss of miRNA biogenesis by NCC-spe...

Abstract Objective To identify causes of technical repeats, determine whether differences exist between mobile and fixed mammography units, evaluate the rate improvement on repeat imaging. Methods IRB approval was obtained for retrospective review Enhancing Quality Using Inspection Program (EQUIP) logs screening repeats performed from March 2017 to December 2018 at a hospital breast imaging cen...