To estimate the turnover of 5-CH,-H4-folate in murine lymphoma cells L1210, LlZlOR (a methotrexate-resistant subline), and L5178Y, suspensions of whole cells were allowed to concentrate 5-[14C]CH,-[9,3’,5’-3H]H4-folate; analysis of cell extracts showed that, for each cell line, 81 to 85 % of the total cell (14C]CH3 groups were transferred to nonfolate compounds within 5 min and 82 to 91% at tim...

Gonadal homogenates of rainbow trout from D(ay) 60, D100 and D200 after fertilization have been incubated in vitro in the presence of dehydroepiandrosterone-3H to demonstrate 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and delta 5,4-isomerase activity, of pregnenolone-3H to assess the synthesis of "progestins" and of androstenedione-3H to determine oestrogen synthesis in the ovaries and th...

quinazolinone backbone is present in a large number of bioactive substances. since remarkable cytotoxic activity is associated with some 4(3h)-quinazolinones, in this study some 4(3h)-quinazolinone were synthesized and screened against hela cells. the synthesis was performed via reaction of anthranilic acid with dicarboxylic anhydrides to produce carboxylic acids derivatives. the products were ...

The objective of this study was to determine the contribution of myocardial triglycerides to overall ATP production in isolated working rat hearts. Endogenous lipid pools were initially prelabeled (pulsed) by perfusing hearts for 60 min with Krebs-Henseleit buffer containing 1.2 mM [1-14C]palmitate. During a subsequent 60-min period (chase), hearts were perfused with either no fat, low fat (0.4...

The synthesis of 2-benzylamino-8-methoxy-4H-chromene-3-carbonitriles 7(a-e) has been realized in four steps, via reductive amination from the 2-amino-8-methoxy-4H-chromene-3-carbonitrile 4 as key intermediate platform with para- and meta-substituted benzaldehydes 5(a-e), good overall yields. physicochemical properties also their aldimines 6(a-e) precursors have determined using Swiss ADME serve...

OBJECTIVES This study examined effects of 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase inhibitor cerivastatin on human saphenous vein (SV), endothelial cells (EC) and smooth muscle cells (SMC). BACKGROUND Venous bypass graft failure involves EC dysfunction and SMC proliferation. Substances that improve EC function and inhibit SMC proliferation would be of clinical relevance. METHODS B...

THE DIHEDRAL ANGLE BETWEEN THE PLANES OF TWO AROMATIC RINGS IN THE TITLE COMPOUND [SYSTEMATIC NAME: 3-benzoyl-5-chloro-pyrimidine-2,4(1H,3H)-dione], C(11)H(7)ClN(2)O(3), is 86.79 (6)°. Centrosymmetric dimers formed by N-H⋯O hydrogen bonds are linked through C-H⋯O inter-actions, forming a two-dimensional network parallel to (10).

In the title complex, [Mn(C(11)H(10)N(3)O(2))(2)]·3H(2)O, the Mn(II) atom is coordinated by four N atoms and two O atoms in a distorted octa-hedral geometry. The mol-ecules are linked together via hydrogen bonds involving the water molecules. One of these is disordered equally over two positions.