AIMS The nucleolus is an important cellular component involved in the biogenesis of the ribosome. This study was performed in order to validate the introduction of the argyrophilic nucleolar organiser region (AgNOR) stain technique, specific for the nucleoli detection, in neuropathological studies on sudden fetal and infant death. METHODS In a wide set of fetuses and infants, aged from 27 ges...

AIM To evaluate the proliferative potential and the cell proliferation rate of odontogenic epithelial cells. MATERIALS AND METHODS Forty-two cases of pericoronal follicles of impacted third molars were submitted to silver impregnation technique for quantification of argyrophilic nucleolar organizer regions (AgNOR) and immunohistochemical staining for EGFR and Ki-67. For AgNOR quantification, ...

In order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-pasture area, in southern Brazil. B chrom...

The objective of this study was to evaluate the cellular proliferative potential of oral lichen planus (OLP) lesions from patients without hepatitis C virus (HCV) by means of AgNOR method, as well as the cellular proliferative potential of the normal oral mucosa from patients with HCV, treated or untreated by interferon and ribavirin. A cross-sectional study was developed to investigate four gr...

The use of Toombak has been reported to play a major role in the etiology of oral cancer in Sudan. The cellular proliferative activity on the oral epithelium of 210 Toombak dippers was assessed by applying the micronuclei frequency, mean argyrophilic nucleolar organizer region (AgNOR) counts, Papanicolaou method, and 1% crystal violet stain. Participants were divided into 3 groups: 200 were app...

Oral Squamous cell carcinoma (OSCC) accounts for over 90% of oral cancer cases and a small percentage of these cases are thought to develop from potentially malignant disorders. Main etiological factor associated with OSCC is smoking. It’s a well known fact that in chronic smokers nuclear alterations are evident. There is a correlation between nucleolar function, size and cell doubling time in ...

The mAgNOR and pAgNOR counts reflecting the number of rDNA genes being transcribed, showed a highly significant increase from control values following administration of 5X, 10X, 15X doses of enrofloxacin in chicken. The maximum increase for both, mAgNOR and pAgNOR was shown by birds receiving 15X dose, sacrificed 48 hr after the last drug injection. The increase of pAgNOR at 5X (24 hr) was not ...

The variations in the numbers of nucleolar organising regions (NORs) among different grades of cervical intraepithelial neoplasia (CIN) were investigated using a silver staining technique. Twenty four biopsy specimens were studied (six normal and six of each of the three grades of CIN) by staining paraffin wax sections using a silver (AgNOR) method that stains the NORS as multiple black dots wi...

S AND ORAL PRESENTATIONS 1. T.Solymosi, V.Toth, M.Bodo, I.Gal. Evaluation of nucleolar organizer region-associated proteins in thyroid cytopathology. Acta Cytol 1993, 37, 622 2. T.Solymosi, I.Gal, I.Györi. Nucleolar organizer regions in colonic cytopreparations. Acta Cytol, 1993, 37, 622-623 3. I.Gal, T.Solymosi, I.Györi. Comparison of nucleolar organizer regions in cytopreparations derived fro...