The International Atomic Energy Agency convened a technical meeting on environmental enteric dysfunction (EED) in Vienna (October 28-30, 2015; https://nucleus.iaea.org/HHW/Nutrition/EED_Technical_Meeting/index.html) to bring together international experts in the fields of EED, nutrition, and stable isotope technologies. Advances in stable isotope-labeling techniques open up new possibilities to...

This paper reports experimental results on the low-temperature (<100 oC) 18O and  2H fractionation of goethite (hematite)-water in a closed system. Both goethite (-FeOOH) and hematite (-Fe2O3) exhibited closer fractionation factor values but the Hematite-Water value is slightly higher (~0.9932) than the Goethite-Water (~0.9924) for the 18O isotope. The average fractionation factor (1000ln...

A combined experimental and theoretical investigation of the deuterium isotope effects on the bacterial luciferase reaction is described. The experimental studies focus on determining if the unusual aldehydic deuterium isotope effect of approximately 1.5 observed in these reactions is an intrinsic isotope effect resulting from a single rate-limiting step or is a composite of multiple rate-limit...

dehydrogenase (Rose et al., 1969). The ,H labelling of ['HI lactate in 2H,0 and the 'H labelling of [2H]lactate in 'H,O has been studied. A comparison of the exchange in whole cells and lysates has also been undertaken. The detailed measurement of exchange kinetics permitted by the n.m.r. technique has revealed the dependence of the exchange on the different enzyme activities and on the triose ...

In comparative proteomic studies, it is important to know the variability associated with sample preparation. In this study, we report the strategy of using SILAC (stable isotope labeling by amino acids in cell culture) to evaluate the effect of the variation in sample preparation for quantitative proteomics. Variability can be measured when equal amounts of light and heavy SILAC samples underg...

We used stable isotope labeling with 4-plex iTRAQ (isobaric tags for relative and absolute quantification) reagents and LC-MS/MS to investigate proteomic changes in the nucleus of activated human CD4(+) cells during the early stages of Th2 cell differentiation. The effects of IL-4 stimulation upon activated naïve CD4(+) cells were measured in the nuclear fractions from 6 and 24 h in three biolo...