The interaction between silver ion and DNA has been followed by submarine gel electrophoresis. When pBR322 plasmid DNA was allowed to interact with silver(I) acetate, it was found to contain Form I and Form II bands whose intensity remained unchanged as the concentration of Ag(+) was increased from 0 to 50 mM. However, the mobility of the bands decreased as the concentration of Ag(+) was increa...

Three Malaysian ginger cultivars (Bukit Tinggi, Tanjung Sepat and Sabah) were collected and examined for genetic polymorphisms using microsatellite DNA primers. The single microsatellite oligonucleotide primers (CATA)5, (GATA)5 and (GAC)6 were used in polymerase chain reactions (PCRs). These PCR reactions produced 7 polymorphic bands with an average of 2.334 polymorphic bands per primer, leadin...

data not shown). Figure 1 demonstrates the two alleles of exon 15 of hMLH1 obtained with and without the modifications described above. The modified technique resulted in unequivocal resolution in the complete absence of artifactual bands. Representative samples of hMLH1 exon 15 were subcloned (TA Cloning® Kit; Invitrogen, San Diego, CA, USA) and sequenced following manufacturer’s instructions ...

DNA based molecular markers allow precise, objective and rapid cultivar identification. In this study, five ISSR and two sets of fluorescence-labeled TP-M13-SSR markers were applied for discrimination of eight closely related wheat cultivars. Five ISSR primers revealed a total of 43 distinct reproducible bands, 29 (67.44%) of the 43 bands were polymorphic. The number of polymorphic bands detect...

Crataegus spp. are used for horticultural and medicinal purposes. The medicinal importance of Crataegus is attributed to components such as flavonoids, procyanidin, aromatic acid, and cardiotonic amines. The genetic relationships among the five Iranian Crataegus species including C. aronia, C. pontica, C. meiery, C. monogyna, and C. pentagyna were assessed using inter simple sequence repeats (IS...

The random amplified polymorphic DNA (RAPD) technique is often criticized because it usually shows low levels of repeatability; thus it can generate spurious bands. These problems can be partially overcome by rigid laboratory protocols and by performing repeatability tests. However, because it is expensive and time-consuming to obtain genetic data twice for all individuals, a few randomly chose...

Grapevine (Vitis vinifera L.) is one of important economic fruit crops found in Salahaldin province \ Iraq. To examine the Molecular characterization and genetic relationships among nine grape varieties by using random amplified polymorphic DNA (RAPD) marker. Fifteen RAPD primers produced band (71, 56%), while two yielded monomorphic bands only. The size fragment ranged between 250-2700 bp, wit...

conclusions torghabeh - shandiz district with a rural texture is endemic for anthroponotic cutaneous leishmaniasis (acl). further studies are recommended to discover the vectors and probable reservoirs in these areas. results of 164 samples obtained from the skin lesions of the patients referred to the torghabe - shandiz health centers, leishmania dna was isolated from 136 smears by pcr method....

The objective of present study was identification of genetic similarity between wild Iran and captive Azerbaijan Pheasant using PCR-RAPD markers. For this purpose, in overall, 28 birds were taken for DNA extraction and subsequently 15 arbitrary primers were applied for PCR-RAPD technique. After electrophoresis, five primers exhibited sufficient variability which yielded overall 65 distinct band...

Isolation of high-quality DNA from plants, especially plants from the Cerrado, is notoriously difficult because of polysaccharides and secondary compounds produced by plants from this biome. DNA isolation and its quality may be compromised by chemical defenses such as tannins and phenols. Quantitative plant defenses tend to have a cumulative effect, increasing in concentration during leaf devel...