The micropropagation of Stevia rebaudiana using stem node segment explants obtained from 2 years-old shrubs were achieved. Cultures were established on Murashige and Skoog (MS) as the medium supplemented with different concentrations of 6-benzylaminopurine (BA) at concentration, 0.0, 0.1 and 0.5 mg/l individually or in combination with Kinetin (Kin) at concentration, 0.0, 0.1 and 0.5 mg/l. The ...

an experiment was conducted to study the micropropagation of satureja khuzistanica. the seeds were planted in 1/2ms medium. after germination and growth of plantlets, they were subcultured in fresh medium. then, they cultured into medium supplemented by different concentrations of ba and kinetin. results showed that, the highest proliferation (2.4 shoots) was observed at concentrations of 0.5 m...

BACKGROUND: In order to investigate the induction of salinity tolerance in CP72-1312 commercial cultivar in tissue culture condition, an experiment was carried out in the tissue culture laboratory of Karoun Agro-Industry Company in 2017 year. OBJECTIVES: At this study we evaluated tolerance of CP73-1312 sugarcane commercial cultivar to salt stress in tiss...

The aim of the study was to determine the optimum concentration of the mare serum (MS) for sheep in vitro oocyte maturation. Sheep ovaries were collected from a local abattoir and transported within 1 h to the laboratory in a warm saline solution (30 – 35C), supplemented with 100 IU penicillin G and 100 g streptomycin sulfate/ml. Cumulus-oocyte complexes (COC’s) were obtained by slicing of foll...

Nodal segments from six years old plants of Bauhinia variegata L. were cultured on Murashige and Skoogs (MS) medium supplemented with different concentration and combinations of BAP and IBA. Explants culture in MS basal medium supplemented with 5mg/1 BAP showed highest rate of shoot multiplication. When in vitro shoots were inoculated on the MS basal medium supplemented with IBA 2mg/l, profusse...

Micropropagation of Kainth using nodal explants was carried out in tissue culture laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2011-13. The effect of various media {1/2 MS (M1), MS (M2) and WPM (M3)} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was studied. Per cent necrotic culture was found to be influenced by...

Withania somnifera (L.) Dunal is high demanding valuable medicinal plants which have pharmaceuticals properties, known for its with anolides. Nodal segment explants of W. somnifera when cultured on MS (Murashige and skoog’s, 1969) medium horizontally containing different concentration(1-3mg/l) of 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ) produced multiple shoots on both axil...

Leaf, seed, and tuber explants of C. latifolia were inoculated on MS medium supplemented with various concentrations of BAP and IBA, alone or in combinations, to achieve in vitro plant regeneration. Subsequently, antioxidant and antibacterial activities were determined from in vitro and in vivo plant developed. No response was observed from seed culture on MS media with various concentrations o...