conclusions our successful extraction of purified lps from e. coli isolates of uti patients’ urine samples can be an important step to understand the uti disease conditions. results the silver-stained gel demonstrated both smooth and rough type lps by showing trail-like band patterns with the presence and lacking o-antigen region, respectively. coomassie blue staining showed no band assuring th...

Background and Objective: Today, one of the problems of health systems is the presence of cockroaches in hospitals as insects that move freely in and out of the hospitals and are infected with pathogenic bacteria. The aim of this study was to identify carbapenem resistance genes in Escherichia coli isolated from Blattella germanica by dot blot assay in Hamadan hospitals in the west of Iran. Me...

enterohemorrhagic escherichia coli (ehec) of the o157:h7 serotype is a worldwide zoonotic pathogen responsible for the majority of severe cases of human ehec disease. the aim of the present study was to investigate the prevalence of e. coli o157: h7/nm in raw meat samples from two provinces of iran. during a period from march 2010 to march 2011. two hundred and ninety five raw meat samples were...

conclusions our study emphasized the increasing role of esbl genes, especially blactx-m15 in epec isolates. results of 42 epec, eight isolates carried the blactx-m1. none of the isolates carried blactx-m2 and blactx-m9. the blactx-m15 variant was identified in all of blactx-m1-positive isolates. furthermore, blashv and blatem genes were detected in 40.5% (n = 17) and 19% (n = 8) of all epec iso...

Background: Although Escherichia coli (E. coli)is a part of intestinal normal microflora of warm-blooded animals, includingpoultry, outbreaks occur in poultry raised below standard sanitation and duringthe course of respiratory or immunosuppressive diseases. Avian pathogenic E.coli (APEC) harbors several genes associated with virulence andpathogenicity. APEC strains are responsible for some dis...

to determine the contamination of traditional cheeses to e. coli o157:h7 this study was conducted in chaharmahal and bakhtiari province. two hundred cheeses samples were obtained randomly and bacteriological examinations were done on all isolates, by culture in selective enrichment media and after that, selective plating. suspected colonies were examined with o and h antisera for identification...

objective(s) extended-spectrum beta-lactamase (esbl) producing bacteria have an important role in nosocomial infections. due to the limited availability of information about the molecular epidemiology of esbl producing bacteria in mashhad, we decided to investigate about tem, ctx and shv esbls among urinary escherichia coli isolates in mashhad, a city in northeast iran. materials and methods   ...