Abstract Background Formation of triploid organism is useful in genetics and breeding. In this study, autotriploid Carassius auratus (3nRR, 3n = 150) was generated from red var. (RCC, 2n 100) (♀) autotetraploid (4nRR, 4n 200) (♂). The female 3nRR produced haploid, diploid eggs, whereas the male infertile. aim present study to explore fertility potential candidate genes 3nRR. Results Gonadal tra...