Simultaneous separation of 37 proteinogenic D/L-amino acids excluding proline with HPLC is generally difficult. In this study, two sets o-phthalaldehyde (OPA)-based fluorescent diastereomers were formed using chiral thiols N-acetyl-L-cysteine (NAC) and N-isobutyryl-L-cysteine (NIBC) independently. It expected that obtained OPA-derivatized D/L show different retention selectivity in reversed pha...

Opa proteins of Neisseria gonorrhoeae bind to CD66 receptors on human phagocytes, thereby inducing efficient uptake of the bacteria in the absence of opsonins. The interaction of Opa proteins and CD66 receptors leads to activation of Src family tyrosine kinases, a process that is of critical importance for the efficient, CD66-mediated internalization. Here we show that during Opa-mediated stimu...

We investigate experimentally the absorptive and dispersive properties of the triple-resonant optical parametric amplifier (OPA) for the degenerate subharmonic field. In the experiment the subharmonic field is utilized as the probe field and the harmonic wave as the pump field. We demonstrate that the electromagnetically induced transparency (EIT)-like effect can be simulated in the triple-reso...

This paper investigates the problem of optimal priority assignment in multiprocessor real-time systems using global fixed task-priority pre-emptive scheduling. Previous work in this area showed that arguably the most effective pseudo-polynomial schedulability tests for global fixed priority pre-emptive scheduling, based on response time analysis, are not compatible with Audsley’s Optimal Priori...

background: as for human trichomoniasis the host-parasite relationship is very complex, and the broad ranges of clinical symptoms are unlikely be attributable to a single pathogenic mechanism. specific random amplified polymorphic dna (rapd) markers of 490 bp, 720 bp and 460 bp using the primers tv-5, opa-6 and opa-11, respectively, were reported. this was the first description of possible ge­n...

PURPOSE To describe a novel excimer laser ablation algorithm, termed optimized prolate ablation (OPA), that uses both topography and ocular aberrometry and maintains or improves the natural corneal shape postoperatively. METHODS A descriptive article outlining the theory behind this OPA algorithm. RESULTS The theoretical changes to the ablation algorithm described in this article will produ...

BACKGROUND The influence of diabetes mellitus on ocular pulse amplitude (OPA), an indirect measure of choroidal perfusion, is unclear. METHODS OPA, using the Langham ocular blood flow (OBF) system, applanation intraocular pressure (IOP), systemic blood pressure (BP), heart rate, and haemoglobin (Hb) A(1c) were measured in patients with insulin dependent diabetes mellitus (IDDM) with no (DR-0,...

We demonstrate the generation of 22.6 μJ of combined energy at 3 μm for sub-300fs pulses at a repetition rate of 1 kHz using a LGSe optical parametric amplifier (OPA). The LGSe OPA is pumped by the 140-fs 1.6 μm pulses from a 300-mW KTA optical parametric chirped pulse amplifier (OPCPA) based on an all-optical synchronization scheme. By using a highly-nonlinear fiber, the output of an erbium-do...

PURPOSE This study compared the measurements of intraocular pressure (IOP) and ocular pulse amplitude (OPA) using the Dynamic Contour Tonometry (DCT) over silicone hydrogel contact lenses of different modulus. Corneal biomechanics were also measured using the Ocular Response Analyzer (ORA). METHODS Forty-seven young (mean age 22.3 years, standard deviation 1.2 years) subjects had IOP, OPA, co...