Small-angle neutron scattering (SANS) has been used to extend the structural characterization of the MS2 phage by examining its physical characteristics in solution. Specifically, the contrast variation technique was employed to determine the molecular weight of the individual components of the MS2 virion (protein shell and genomic RNA) and the spatial relationship of the genomic RNA to its pro...

Infection of cells transduced with a lentiviral vector by human immunodeficiency virus (HIV) could lead to packaging of the lentiviral vector RNA into HIV particles and unintended transfer of the vector. To prevent this, the Rev-responsive element (RRE) of an HIV-1 vector was functionally replaced by a heterologous RNA element (MS2). Providing Rev fused to an MS2 binding protein allowed efficie...

Adenosine deaminases acting on RNA (ADARs) have double-stranded binding domains and a deaminase domain (DD). We used the MS2 system specific guide RNAs to direct ADAR1-DD target adenosines in mRNA encoding-enhanced green fluorescence protein. Using this transfected HEK-293 cells, we evaluated effects of changing length position efficiency conversion amber (TAG) ochre (TAA) stop codons tryptopha...

background and objectives: food contamination by aflatoxins is an important food safety concern for agricultural products. in order to identify and develop novel antifungal agents, several plant extracts and isolated compounds have been evaluated for their bioactivities. anti-infectious activity of piper betle used in traditional medicine of malaysia has been reported previously. materials and ...

Cells possess a number of active segregation machineries for both chromosomal and large extrachromosomal DNA elements to avoid stochastic loss during cell division. In contrast, system that can be exploited active, general RNA molecules including mRNAs or self-replicating constructs are currently lacking. Here, we present an artificial derived from the bacterial type II ParMRC plasmid coliphage...