Journal:
:iranian journal of basic medical sciences
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nasrin heydari department of genetics and molecular biology, school of medicine, isfahan university of medical sciences, isfahan, iran
laleh shariati department of genetics and molecular biology, school of medicine, isfahan university of medical sciences, isfahan, iran
hossein khanahmad department of genetics and molecular biology, school of medicine, isfahan university of medical sciences, isfahan, iran
pediatric inherited diseases research center, research institute for primordial prevention of non-communicable disease, isfahan university of medical sciences, isfahan, iran
zahra hejazi department of genetics and molecular biology, school of medicine, isfahan university of medical sciences, isfahan, iran
mansoureh shahbazi department of genetics and molecular biology, school of medicine, isfahan university of medical sciences, isfahan, iran
objective(s): β-thalassemia is one of the most common genetic disorders in the world. as one of the promising treatment strategies, fetal hemoglobin (hb f) can be induced. the present study was an attempt to reactivate the γ-globin gene by introducing a gene construct containing klf1 binding sites to the k562 cell line. materials and methods: a plasmid containing a 192 bp sequence with two repe...