Alu repeats or Line-1-ORF2 (ORF2) inhibit expression of the green fluorescent protein (GFP) gene when inserted downstream of this gene in the vector pEGFP-C1. In this work, we studied cis-acting elements that eliminated the repression of GFP gene expression induced by Alu and ORF2 and sequence characteristics of these elements. We found that sense and antisense PolyA of simian virus 40 (SV40Pol...

The urokinase-type plasminogen activator (uPA) plays an important role in cellular invasion. By using the downstream part of a 74 bp DNA region called the cooperation mediator (COM) of the uPA promoter as a bait sequence in the yeast one-hybrid screen, a gene called PBK1 was previously cloned from the cDNA library of the 95D lung cancer cell strain. In this study, the intracellular distribution...

BACKGROUND Human interacting protein X1 (PinX1) has been identified as a critical telomerase inhibitor and proposed to be a putative tumor suppressor gene. Loss of PinX1 has been found in a large variety of malignancies, however, its function in inhibiting telomerase activity of tumor cells is not well documented. Here we show that PinX1 is essential for down-regulation telomerase activity of n...

HARTAWAN, R., K. ROBINSON, T. MAHONY and J. MEERS. 2011. In vitro expression of native H5 and N1 genes of avian influenza virus by using Green Fluorescent Protein as reporter. JITV 16(3): 234-241. The hemagglutinin and neuraminidase are important immunogen of avian influenza virus that are suitable for recombinant experimentation. However, both genes have been experienced rapid mutation resulti...

Evidence suggests that Pin2/TRF1-interacting protein X1 (PinX1) inhibits telomerase activity in many types of cancer cells. G-patch is a motif in the PinX1 protein; however, the function of G-patch in colorectal cancer cells has not been definitively elucidated. The present study investigated the antitumor activities of different PinX1 fragments in vitro, and explored the molecular mechanisms r...

An eukaryotic expression system of Congjiang pigs IFN-λ1 was constructed to obtain its in CHO-K1 cells and the inhibition effect pig on PRRSV proliferation verified. The plasmid pEGFP-PoIFN-λ1 from gene fragment transfected into cells. Expression detected by fluorescence microscopy Western blotting. influence assessed. results study showed that recombinant correctly. After transfection, green f...

Toxoplasma gondii cathepsin C proteases (TgCPC1, 2, and 3) are important for the growth and survival of T. gondii. In the present study, B-cell and T-cell epitopes of TgCPC1 were predicted using DNAstar and the Immune Epitope Database. A TgCPC1 DNA vaccine was constructed, and its ability to induce protective immune responses against toxoplasmosis in BALB/c mice was evaluated in the presence or...