We have used monoclonal antibody binding, measured by radioimmunoassay, fluorescence flow cytometry, and ultrastructural immunocytochemistry, to measure expression of Ia antigens on cultured human umbilical vein endothelial (HUVE) cells. Under standard culture conditions, HUVE cells do not express Ia antigens. However, treatment of primary HUVE cultures with phytohemagglutinin induces the expre...

Cysteines are thought integral to conformational epitopes of islet antigen-2 (IA-2) autoantibodies (IA-2A), possibly through disulfide bond formation. We therefore investigated which cysteines are critical to IA-2A binding in patients with newly diagnosed type 1 diabetes. All 10 cysteines in the intracellular domain of IA-2 were modified to serine by site-directed mutagenesis, and the effects o...

OBJECTIVE To determine the recurrence risk of ictal asystole (IA) and its determining factors in people with epilepsy. METHODS We performed a systematic review of published cases with IA in 3 databases and additionally searched our local database for patients with multiple seizures simultaneously recorded with ECG and EEG and at least one IA. IA recurrence risk was estimated by including all ...

OBJECTIVE To report the FLEXX trial, the first well-controlled study assessing the safety and efficacy of Euflexxa (1% sodium hyaluronate; IA-BioHA) therapy for knee osteoarthritis (OA) at 26 weeks. METHODS This was a randomized, double-blind, multicenter, saline-controlled study. Subjects with chronic knee OA were randomized to 3 weekly intra-articular (IA) injections of either buffered sali...

Steganography embeds a secret message in an innocuous cover-object. This paper identifies three cover-specific redundancies of executable programs and presents steganographic techniques to exploit these redundancies. A general framework to evaluate the stealth of the proposed techniques is introduced and applied on an implementation for the IA-32 architecture. This evaluation proves that, where...

0272-1732/00/$10.00  2000 IEEE The press and the technical community have generated much excitement and speculation about the IA-64 instruction set and the Itanium processor. Intel and HewlettPackard have rolled out (one instruction at a time) the instruction set. Intel is rolling out (one transistor at a time) the Itanium processor and other platform components. This special issue provides th...