S-Adenosylhomocysteine (AdoHcy) hydrolase is irreversibly inactivated by (E)-5',6'-didehydro-6'-deoxy-6'-halohomoadenosines (EDDHHAs, halogen = I, Br, and Cl). The inactivation is concomitant with the reduction of the enzyme-bound NAD+ (E.NAD+) to NADH, the release of halide ion, and the formation of adenine (Ade) from the EDDHHAs. The mechanism of this inactivation involves two catalytic pathw...

BACKGROUND The root of Angelica dahurica Bentham et Hooker (Umbelliferae) has been used as a traditional medicine for colds, headache, dizziness, toothache, supraorbital pain, nasal congestion, acne, ulcer, carbuncle, and rheumatism in China, Japan, and Korea. Interestingly, it has been used in the treatment of vascular diseases including hypertension. The aim of this study was to provide pharm...

This paper describes the collaboration of Brigham and Women’s Hospital and Regenstrief Institute to develop a computerized adverse drug event (ADE) monitor using electronic medical records from outpatient practices. We describe the steps involved in ADE monitor development and rule validation at large outpatient practices at Boston and Indianapolis. The final standard rule set adopted by both p...

BACKGROUND AND OBJECTIVES Outpatient adverse drug events (ADEs) can result in serious outcomes requiring emergency department (ED) visits and hospitalizations. The incidence and severity of ADEs in children with complex chronic conditions (CCCs), who often take multiple medications, is unknown. We sought to describe the characteristics of ADE-related ED visits, including association with CCC st...

Respiratory syncytial virus (RSV) is the leading cause of severe respiratory illness in infants. At this young age, infants typically depend on maternally transferred antibodies (matAbs) and their innate immune system for protection against infections. RSV-specific matAbs are thought to protect from severe illness, yet severe RSV disease occurs mainly below 6 months of age, when neutralizing ma...

IMPORTANCE In 2011, an estimated 26.8 million US adults used prescription medications for mental illness. OBJECTIVE To estimate the numbers and rates of adverse drug event (ADE) emergency department (ED) visits involving psychiatric medications among US adults between January 1, 2009, and December 31, 2011. DESIGN AND SETTING Descriptive analyses of active, nationally representative surveil...

We have carried out a picosecond fluorescence study of holo- and apoazurins of Pseudomonas aeruginosa (azurin Pae), Alcaligenes faecilis (azurin Afe), and Alcaligenes denitrificans (azurin Ade). Azurin Pae contains a single, buried tryptophyl residue; azurin Afe, a single surface tryptophyl residue; and azurin Ade, tryptophyl residues in both environments. From anisotropy measurements we conclu...

This paper reviews extant agent applications and describes the Agent Development Environment (ADE) toolkit. ADE is the integrated development environment to design, develop, debug, simulate and deploy agents. ADE supports the development of multi-agent applications capable of running on a single machine or on a distributed network. ADE has been used to build commercial applications in the area ...

Concern that ADE of HIV infection could occur in vivo, as a result of HIV immunization, has arisen for several reasons. Immune-mediated disease enhancement occurs in several human and animal viral diseases, including lentiviral diseases. Tropism for host M/M cells is a common characteristic in these diseases. Sera from naturally infected, and possibly HIV-immunized, individuals have been shown ...