Erythrocytes were fractionated, by centrifugation, on the basis of cell density, which is proportional to cell age. Glycated hemoglobin (Hb A1c) in the fractionated cells was measured and compared with the Hb A1c in whole blood and with the fructosamine concentrations in plasma of normal and diabetic subjects. In normal subjects, Hb A1c increased according to the age of the erythrocytes, but hy...

blood specimens from 22 insulin dependent diabetic patient and 17 nontliabetic subject were collected im edta- tubes. quantitation of hb-al was performed by columne chromatography. the glycosylated hemoglobin component is increased in diabetic patient and found a significant correlation between hb-al concentration and blood glucose levels in all diabetic patients. the mean hb-al valve for diabe...

Hb F, some of which is glycated (2), accounts for the majority (70%–95%) of Hb species in newborns. About 5%– 30% is Hb A, with none to trace amounts of Hb A1c. This patient’s sample was collected after transfusion of 2 aliquots of red blood cells from a single donor. The donor’s blood contained 12.6% Hb A1c. Concentrated glucose in blood storage media increases donor Hb A1c only minimally (3, ...

Give a brief description of Haemoglobin (Hb):  Hb is made up of Four Subunits (Tetramer) held together by multiple non-covalent interactions  Each subunit (Monomer) consist of: Haem (Ferro-Protoporphyrin) and Globin protein  Haem consist of a Protoporphyrin IX and Ferrous ion (Fe 2 )  Globin protein folds around the Haem group forming a hydrophobic pocket that protects the Haem, which serve...

BACKGROUND Estimating blood loss in trauma patients usually involves the determination of hematocrit (Ht) or hemoglobin (Hb). However, in trauma patients, a poorly substantiated habit exists to determine both Ht and Hb in assessing acute blood loss. This suggests that Ht and Hb provide different information. Moreover, a survey of the literature showed a significant association of the subject tr...

Haptoglobin (Hp) counterbalances the adverse effects of extra-erythrocytic hemoglobin (Hb) by trapping ?? dimers Hb in bloodstream. In turn, Hp:Hb complexes display Hb-like reactivity. Here, kinetics NO dissociation from ferrous nitrosylated (i.e., Hp1–1:Hb(II)-NO and Hp2–2:Hb(II)-NO, respectively) are reported at pH 7.0 20.0 °C. Hp:Hb(II)-NO has been followed replacing with CO. Denitrosylation...

When low levels of gaseous nitric oxide (NO) are equilibrated with deoxygenated Hb, all NO added can be accounted for in terms of hexacoordinate and pentacoordinate forms of NO-Hb, despite recent reports on NO disappearance from heme groups to form nitroxyl anions or S-nitrosated Hb at low ratios of NO to Hb. We demonstrate that a fraction of the spectral signature of fully nitrosylated (largel...

Complete automation of high-performance liquid chromatography (HPLC) for determination of hemoglobin F (%Hb F) and hemoglobin A1c (%Hb A1c) levels has made this procedure available in many clinical laboratories. However, the physiological changes in %Hb F during pregnancy and the effects of physiological and supraphysiological levels of %Hb A1c on measurement of %Hb F have not been studied exte...