Chronic myeloid leukaemia (CML) is a form of leukaemia derived from the myeloid cell lineage. Imatinib mesylate, the breakpoint cluster region-abelson murine leukeamia kinase inhibitor, is a specific reagent used in the clinical treatment of CML. The DNA topoisomerase II inhibitor, etoposide, is also employed as a therapeutic, though it is used to a lesser extent. The present study aims to eval...

Previous analysis of the hemoglobin phenotype of the K562 human erythroleukemia cell line showed regulated expression of the e, J-. y, a-, and &globin genes. Expression of the p-globin genes has not been previously detected in this cell line. In this report, we describe the isolation of a variant of the K562 cell line that actively expresses @globin messenger RNA (mRNA) and polypeptide and show...

To investigate the effect of inhibition of Na(+)/H(+) exchanger isoform1 (NHE1) on K562 leukemia-driven angiogenesis, the selective NHE1 inhibitor cariporide was used. Cariporide treatment of K562 resulted in a decrease in pHi and down-regulation of VEGF secretion. The proliferation, migration and in vitro tube formation of human umbilical vein endothelial cells was decreased in cariporide trea...

انتقال ویروس کرونا به داخل سلول از طریق اسپایک‌های انجام می‌شود پس یکی بهترین راه‌های مقابله با سلول‌های بدن، ایجاد اختلال در روند عملکرد است. بنابراین این تحقیق، چگونگی جذب و انرژی پرتوهای رادیواکتیو سارس کووید2، مرس کووید، یوکی کووید توسط ابزار جینت4- دی اِن اِی شبیه‌سازی شده نتایج حاصل پرتودهی اسپایک‌ها یکدیگر مقایسه آن‌جایی که عوامل کاهش ماندگاری می‌باشد، پژوهش سعی است اسپایک ویروس­‌ها به‌وسی...

BACKGROUND The regulation of growth and apoptosis in K562 cells by human bone marrow mesenchymal stem cells (MSCs) from leukemia patients was investigated. METHODS K562 cells were cocultured with leukemic MSCs under serum deprivation. Cell Counting Kit-8 (CCK-8), PI staining, Annexin V/PI binding and FACS assays were used to investigate cell proliferation, cell cycle status, and apoptosis of ...

Clinical studies have shown that the tyrosine kinase inhibitor STI571 effectively controls BCR-ABL-positive chronic myelogenous leukemia (CML). However, disease progression while on STI571 therapy has been reported, suggesting de novo or intrinsic resistance to BCR-ABL-targeted therapy. To investigate possible mediators of acquired STI571 resistance, K562 cells resistant to 5 microM STI571 (K56...

To explore the effects of resveratrol in a human myelogenous leukemia cell line K562 and its potential molecular mechanisms. The anti-proliferation effect of resveratrol-induced apoptosis on K562 cells were detected using MTT assay. Western blotting was performed for detecting changes of SphK1 expression in total cell protein and membrane/cytosol protein in K562 cells respectively after exposur...