Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported bilayers (SLBs), mimicking composition HeLa cell plasma membrane. We charac...

This study aimed to investigate the effect of fullerenol combined with cisplatin on the proliferation of cervical cancer HeLa cells in order to provide new ideas and laboratory theoretical basis for the clinical treatment of cervical cancer. Cervical cancer cell line HeLa cells in vitro were treated with different concentrations of fullerenol, different concentrations of cisplatin, and differen...

The effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport were examined in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP. Sensitivity to mitoxantrone, an ABCG2/BCRP substrate, in HeLa/SN100 cells was significantly reversed by the coexistence of the calcium antagonists, except for diltiazem a...

Human adenovirus type 5 enhances the thymidine kinase activity of KB cells but does not induce the enzyme in kinase-deficient HeLa (BU25) cells. Vaccinia induces thymidine kinase activity in both KB and HeLa (BU25) cells. Human adenovirus types 2, 4, 7, and 12 also fail to induce the enzyme in HeLa (BU25) cells. Vaccinia replicates equally well in the presence or absence of HATG (hypoxanthine-a...

Newly synthesized histone H4 is deposited in a diacetylated isoform in a wide variety of organisms. In Tetrahymena a specific pair of residues, lysines 4 and 11, have been shown to undergo this modification in vivo. In this report, we demonstrate that the analogous residues, lysines 5 and 12, are acetylated in Drosophila and HeLa H4. These data strongly suggest that deposition-related acetylati...

BACKGROUND/AIM The claudin family of proteins are key constituents of tight junctions and the aberrant expression of these proteins can contribute to de-stabilisation of tight junctions and thus to loss of cell polarity and cohesion. Increased expression of claudin-1 and claudin-7 has been observed in pre-invasive cervical lesions and cervical carcinomas. The present study attempted to assess t...

OBJECTIVE To investigate the effect of recombinant human p53 adenovirus injection combined with paclitaxel on human cervical cancer HeLa cell proliferation, apoptosis and expression of vascular endothelial growth factor (VEGF). MATERIALS AND METHODS Detection of effects of paclitaxel, rAd-p53, and drug combination on the proliferation of HeLa cells by MTT method. Detection of effects of pacli...

The horse-adapted virulent Bucyrus (VB) strain of equine arteritis virus (EAV) established persistent infection in high-passage-number human cervix cells (HeLa-H cells; passages 170 to 221) but not in low-passage-number human cervix cells (HeLa-L cells; passages 95 to 115) or in several other cell lines that were evaluated. However, virus recovered from the 80th passage of the persistently infe...