Diffuse-reflectance laser flash photolysis has been used to record transient spectra and decay kinetics of the photodynamic therapy sensitizer disulfonated aluminium phthalocyanine in two murine cancer cell lines, P815 derived from white mouse mast cells, and EL4, a lymphoblast derived from black mouse lymphocytes. In contrast to results with bacterial cells and yeasts, no transient other than ...

Immunoaffinity-purified interleukin 2 (IL2) stimulated proliferation of large but not small B cells. Stimulation was observed even when B cells were cultured at very low cell densities (3 X 10(4) per microwell containing 0.2 ml of medium). Addition of small numbers of purified splenic T cells did not enhance the IL2-induced B-cell proliferative response. These results suggest that IL2 was not o...

Reports on the bacterial utilization of u-arabinose have shown two pathways of dissimilation. The first, reported by Cohen (l), demonstrated that Escherichia coli first isomerized n-arabinose to n-ribulose, which was then phosphorylated. A later report by Doudoroff et al. (2) showed that Pseudomonas saccharophi&a oxidized n-arabinose to n-arabonolactone, which was subsequently hydrolyzed to the...

With the use of in vitro methods and cell lines, functional aspects of apoptosis in the Xenopus laevis B3/B7 and mouse EL4 thymoma cell lines are revealed. Moreover, by using information gleaned from digital imaging and immunocytochemistry, changes in locations of key proteins implicated in apoptotic anti-cancer responses, e.g. p53 and Mdm2, are shown. Suggestions are offered as to what these r...

We report for the first time that IFNG gene expression requires high mobility group (HMG)A1, the architectural transcription factor mediating enhanceosome formation. This finding is supported by our direct studies of T cells isolated from the HMGA1-transgenic mice displaying an up-regulation of IFN-gamma production and of HMGA1-deficient mice exhibited a decreased IFN-gamma induction. In parall...

We report for the first time that IFNG gene expression requires high mobility group (HMG)A1, the architectural transcription factor mediating enhanceosome formation. This finding is supported by our direct studies of T cells isolated from the HMGA1-transgenic mice displaying an up-regulation of IFN-c production and of HMGA1-deficient mice exhibited a decreased IFN-c induction. In parallel trans...