Source/description : Genomic DNA was extracted from a whole Pacific oyster (C. gigas) after grinding in liquid nitrogen. Purified DNA was then digested by a mix of 3 restriction enzymes (AluI, RsaI, HaeIII). DNA fragments ranging from 250 to 500 bp were size-selected by agarose gel electrophoresis and ligated into the dephosphorylated blunt-ended SmaI-linearised pBKS2 plasmid (Stratagene Clonin...

The objective of this work was to screen the EU-regulated lipophilic and cyclic imine toxins in four bivalve species (Atrina vexillum, Pinctada imbricata, Anadara antiquata, Saccostrea Cucculata) from Mozambican coast Indian Ocean. Toxins were extracted analyzed according EU reference method for determination shellfish via LC–MS/MS, but no regulated found species. However, pinnatoxins (PnTX G, ...

The wellbeing of marine organisms is connected to their microbiome. Oysters are a vital food source and provide ecological services, yet little known about how climate change such as ocean acidification warming will affect We exposed the Sydney rock oyster, Saccostrea glomerata, orthogonal combinations temperature (24, 28 °C) pCO2 (400 1000 ?atm) for eight weeks used amplicon sequencing 16S rRN...