نتایج جستجو برای: ثبت whole cell patch clamp

تعداد نتایج: 1972952  

2009
Michael Rust

29 THE 4TH ANNUAL IEEE INTERnational Conference on Nano/Microengineered Molecular Systems (IEEE-NEMS) was held at the Sheraton Dameisha Resort in Shenzhen, China, 5–8 January 2009. More than 300 papers were presented by leading micro/nanotechnology researchers in academia and industry from around the globe. Topical areas of the conference included microelectro mechanical systems (MEMS), BioMEMS...

Journal: :American journal of physiology. Renal physiology 1998
Dragana M Filipovic Olugbenga A Adebanjo Mone Zaidi W Brian Reeves

Extracellular ATP affects a wide variety of cells via purinergic membrane receptors. One class of purinergic receptors, P2X, consists of ATP-gated, calcium-permeable, cation-selective channels. We performed whole cell patch-clamp studies, intracellular calcium concentration ([Ca2+]i) measurements, and reverse transcription-polymerase chain reaction (RT-PCR) to determine whether P2X receptors ar...

Journal: :The European journal of neuroscience 1996
P rossi E D'Angelo V Taglietti

UNLABELLED Whole-cell patch-clamp recordings in rat cerebellar slices were used to investigate the effect of metabotropic glutamate receptor activation on mossy fibre-granule cell synaptic transmission. Transient application of 20 microM 1S, 3R-aminocyclopentane-1, 3-dicarboxylic acid simultaneously with low-frequency NMDA receptor activation induced long-lasting non-decremental potentiation of...

Journal: :cell journal 0
gholamhossein meftahi zohreh ghotbedin mohammad javad eslamizade narges hosseinmardi mahyar janahmadi

objective: resveratrol, a phytoalexin, has a wide range of desirable biological actions. despite a growing body of evidence indicating that resveratrol induces changes in neuronal function, little effort, if any, has been made to investigate the cellular effect of resveratrol treatment on intrinsic neuronal properties. materials and methods: this experimental study was performed to examine the ...

Journal: :The American journal of physiology 1990
B Schubert A M Vandongen G E Kirsch A M Brown

The mechanism by which the beta-adrenergic agonist isoproterenol (ISO) modulates voltage-dependent cardiac Na+ currents (INa) was studied in single ventricular myocytes of neonatal rat using the gigaseal patch-clamp technique. ISO inhibited INa reversibly, making the effect readily distinguishable from the monotonic decrease of INa caused by the shift in gating that customarily occurs during wh...

Journal: :Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al 2006
Leonor M E Ituarte Teresa B Viera Teobaldo A Saldeña Juan C de Rosas Mabel Fóscolo Jorge E Ibáñez Fernando D Saraví

Electrophysiological events occur early after fertilization, along with changes in intracellular Ca2+ concentration. Passive electrical parameters were determined in golden hamster oocytes by whole cell patch-clamp method. In separate experiments the effect of 4-aminopyridine on resting oocytes was tested. The single-channel patch clamp configuration was employed to assess the electrical respon...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 1998
L S Dove L C Abbott W H Griffith

The leaner (tgla) mutation in mice results in severe ataxia and an overt neurodegeneration of the cerebellum. Positional cloning has revealed that the tgla mutation occurs in a gene encoding the voltage-activated calcium channel alpha1A subunit. The alpha1A subunit is highly expressed in the cerebellum and is thought to be the pore-forming subunit of P- and Q-type calcium channels. In this stud...

Journal: :Journal of neurophysiology 1998
G S Hollrigel S T Ross I Soltesz

Whole cell patch-clamp recordings were used to investigate the properties of the gamma-aminobutyric acid type A (GABAA) receptor-mediated spontaneous synaptic events in immature granule cells of the developing, early postnatal day (P0-P6) rat dentate gyrus. With Cs-gluconate-filled whole cell patch pipettes at 0 mV in control medium, spontaneous inhibitory postsynaptic currents (sIPSCs) occurre...

2004
Ben Ovryn Xiang Li Hillel Chiel Stefan Herlitze

A preliminary result supports the feasibility of using visible light to modulate the membrane potential of a cell. Human embryonic kidney cells (HEK293) were transfected with vertebrate rhodopsin and a gradient inward rectifying potassium (GIRK) channel. Whole cell patch clamp recordings of HEK293 cells exposed to 9-cis retinal showed that illumination increases the potassium current compared w...

2010
Matthias Braun Reshma Ramracheya Martin Bengtsson Anne Clark Jonathan N. Walker Paul R. Johnson Patrik Rorsman

OBJECTIVE Paracrine signaling via gamma-aminobutyric acid (GABA) and GABA(A) receptors (GABA(A)Rs) has been documented in rodent islets. Here we have studied the importance of GABAergic signaling in human pancreatic islets. RESEARCH DESIGN AND METHODS Expression of GABA(A)Rs in islet cells was investigated by quantitative PCR, immunohistochemistry, and patch-clamp experiments. Hormone release...

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