We developed a PCR-based assay to quantify trichothecene-producing Fusarium based on primers derived from the trichodiene synthase gene (Tri5). The primers were tested against a range of fusarium head blight (FHB) (also known as scab) pathogens and found to amplify specifically a 260-bp product from 25 isolates belonging to six trichothecene-producing Fusarium species. Amounts of the trichothec...

In South Africa, the main causal organisms of Fusarium head blight (FHB) are Gibberella zeae (=Fusarium graminearum), F. culmorum and F. crookwellense. Fusarium graminearum and F. culmorum are associated with warmer regions, and F. crookwellense with cooler regions. Sporadic FHB outbreaks occur principally in the irrigation areas of the country. In favourable years significant damage is caused....

The abbreviations used are: Cygb, cytoglobin; FHb, flavohemoglobin; GCS, globin-coupled sensor; Hb, hemoglobin; HBL, hexagonal bilayer; Mb, myoglobin; MCP, methyl accepting chemotaxis protein; Ngb, neuroglobin; NS, nonsymbiotic; RBC, red blood cell; SD, single domain; SDF, SD FHb-like; SDS, http://www.jbc.org/cgi/doi/10.1074/jbc.R700029200 The latest version is at JBC Papers in Press. Published...

In this study, conducted for three years on eleven malting barley varieties cultivated in central Italy, the incidence of different mycotoxigenic fungal genera, the identification of the Fusarium species associated with the Fusarium Head Blight (FHB) complex, and kernels contamination with deoxynivalenol (DON) and T-2 mycotoxins were determined. The influence of climatic conditions on Fusarium ...

Triticale (xTriticosecale Wittmack) is an important feed crop which suffers severe yield, grade and end-use quality losses due to Fusarium head blight (FHB). Development of resistant triticale cultivars is hindered by lack of effective genetic resistance sources. To dissect FHB resistance, a doubled haploid spring triticale population produced from the cross TMP16315/AC Ultima using a microspor...

Fusarium head blight (FHB) significantly reduces grain yield and quality. Identification of genes that govern FHB resistance/susceptibility will facilitate biotechnology-assisted development of superior wheat varieties with FHB resistance. Our study on global expression profiling using microarray and real-time PCR identified three genes that are significantly highly expressed (up-regulated) in ...

The fungi of the genus Fusarium cause Fusarium head blight (FHB), a devastating disease that reduces grain yield and quality. They also produce mycotoxins which may pose a serious threat to human and animal health. This study investigated the effects of NPK fertilisation, foliar application of Cu, Zn, and Mn, applied separately and in combination, and of the Nano-Gro® organic growth stimulator ...

Fusarium head blight (FHB) causes significant grain loss and contamination of grains with harmful mycotoxins, especially deoxynivalenol (DON). Fusarium resistance and DON accumulation have been extensively investigated in various cultivars; however, the level of DON-3-O-glucoside (D3G) has not been as carefully studied. In this study, we measured accumulated DON and D3G levels in CIMMYT wheat e...

Fusarium Head Blight (FHB) or ‘scab’ is a very destructive disease that affects wheat crops. Recent research has resulted in accurate weather-driven models that estimate the probability of an FHB epidemic based on experiments. However, these predictions ignore two crucial aspects of FHB epidemics: (1) An epidemic is very unlikely to occur unless the plants are flowering, and (2) FHB spreads by ...

BACKGROUND Fusarium head blight (FHB), mainly caused by Fusarium graminearum, substantially reduces wheat grain yield and quality worldwide. Proteins play important roles in defense against the fungal infection. This study characterized differentially expressed proteins between near-isogenic lines (NILs) contrasting in alleles of Fhb1, a major FHB resistance gene in wheat, to identify proteins ...