The HER2/neu oncogene has been reported to be amplified in > 20% of invasive ductal carcinomas. In order to investigate the HER2/neu status in pure populations of breast cancer cells, a laser capture microdissection (LCM) system was used. Formalin-fixed paraffin-embedded breast tissue areas corresponding to normal ducts, ductal carcinoma in situ (DCIS) and invasive ductal carcinoma (IDC) were m...

Laser capture microdissection (LCM)-enabled region-specific tissue analyses are critical to better understand complex multicellular processes. However, current proteomics workflows entail several manual sample preparation steps and are challenged by the microscopic mass-limited samples generated by LCM, impacting measurement robustness, quantification and throughput. Here, we coupled LCM with a...

At varying intervals after immunizing infections of adult BALB/c mice with lymphocytic choriomeningitis (LCM) virus, splenic lymphocytes were tested for their ability to either elicit acute LCM or protect against lethal intracerebral LCM virus challenge when transferred to syngeneic recipients that were, respectively, virus carriers induced by cyclophosphamide-induced immunosuppression and norm...

Objectives: Dynamic Neuromuscular Stabilization (DNS) approach evaluates and activates the spinal stabilizers to optimize the performance of posture and respiratory system. This study investigated the effects of DNS breathing exercises on upper and lower chest wall mobility (UCM and LCM), trunk extensor endurance, and thoracic kyphosis in a group of sedentary students with poor posture. Method...

  Background: Polyinosinic:polycytidylic acid (Poly-IC) has been used as a viral stimulus to mimic in vivo and in vitro infection induced by some viruses. Objective: To determine whether non-viable Lactobacillus casei CRL431 (LcM) can modulate the immune response induced by Poly I:C in co-culture models of peripheral blood mononuclear cells (PBMC) and A549 cells. Methods: T and NK cell activati...

The ability of cells to proliferate in the presence of low extracellular calcium levels has been proposed as a marker for malignancy. To further evaluate this marker, the effects of extracellular calcium deprivation on proliferation and morphology in culture were examined in eight tumorigenic lines derived from human cancers. With calcium reduced from 1.8 mM (HCM) to 0.02 mM (LCM), the colony-f...

A3 Start with a finite sequence a1, a2, . . . , an of positive integers. If possible, choose two indices j < k such that aj does not divide ak, and replace aj and ak by gcd(aj , ak) and lcm(aj , ak), respectively. Prove that if this process is repeated, it must eventually stop and the final sequence does not depend on the choices made. (Note: gcd means greatest common divisor and lcm means leas...

Using a simple basis of rational polynomial-like functions P0, . . . , Pn−1 for the free module of functions Z/nZ → Z/mZ, we characterize the subfamily of congruence preserving functions as the set of linear combinations of the products lcm(k)Pk where lcm(k) is the least common multiple of 2, . . . , k (viewed in Z/mZ). As a consequence, when n ≥ m, the number of such functions is independent o...

Division of Physical Chemistry, Institute of Advanced Materials, Physicochemical Processes, Nanotechnology and Microsystems (IAMPPNM), NCSR Demokritos, 15310 Aghia Paraskevi, Attikis, Athens, Greece LCM – Laboratory of Catalysis and Materials – Associate Laboratory LSRE/LCM, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal Grupo de Fotocatálisis y ...

AIM This study investigated the influence of different composite resin organic matrix (methacrylate - Filtek Z350 XT and silorane - Filtek P90) on light energy transmission through the composite and bottom/top rate. MATERIALS AND METHODS A light-emitting diode (New Blue Phase), light-curing unit was used with different photoactivation protocols (high-continuous mode - HCM, 1400 mW/cm2 for 20 ...