The diffusion of fluoresceinated probes inside single collagen fibrils was investigated by imaging the migration of fluorescence along the fibrils in oil and by monitoring fluorescence recovery after photobleaching (FRAP). Probes were excluded from the fibrils according to their size. Probes that were not excluded diffused in the fibrils, but FRAP occurred 6 x 10(-4) times more slowly than in w...

Plastid stromules are membrane-bound protrusions of the plastid envelope that contain soluble stroma. Stromules are often found connecting plastids within a cell and fluorescence recovery after photobleaching (FRAP) experiments have demonstrated that green fluorescent protein (GFP) can move between plastids via these connections. In this report, the ability of endogenous plastid proteins to tra...

Salah satu tanaman yang mempunyai nilai ekonomis tinggi yaitu rosemary (Rosemarinus officinalis L.). Tanaman ini aroma dan rasa khas banyak mengandung senyawa dapat dimanfaatkan sebagai antioksidan, antijamur, antivirus, antibakteri, antitumor, antitrombotik, antidepresan. Pemanfaatan terutama ekstrak daunnya dilakukan dengan terlebih dahulu melakukan pengujian terhadap tersebut, sehingga tujua...

BACKGROUND & AIM n-3 fatty acid intake has been associated with inflammatory benefits in cardiovascular disease (CVD). Functionalising meat may be of great interest. The aim of the present study was to assess the effect of functional meat containing n-3 and rosemary extract on inflammatory and oxidative status markers in subjects with risk for CVD. METHODS AND RESULTS A randomised, double-bli...

Fluorescent lipid and phospholipid probes were incorporated at 4 degrees C into soybean protoplasts prepared from cultured soybean (SB-1) cells. Fluorescence microscopy showed that the plasma membrane as well as the nucleus were labeled. Fluorescence redistribution after photobleaching (FRAP) analysis was performed on these cells at 18 degrees C to monitor the lateral mobility of the incorporat...

In neurons L-type calcium currents function in gene regulation and synaptic plasticity, while excessive calcium influx leads to excitotoxicity and neurodegeneration. The major neuronal Ca(V)1.2 L-type channels are localized in clusters in dendritic shafts and spines. Whereas Ca(V)1.2 clusters remain stable during NMDA-induced synaptic depression, L-type calcium currents are rapidly downregulate...

cigarette smoke is injurious to both oral cavity and internal body environment. saliva is the firstbody fluid to encounter the dangerous smoke. the aim of our present study was, therefore, toevaluate the influence of smoking on the antioxidant activity of saliva of healthy smokers.antioxidant capacity of saliva was determined using different methods in the centrifuged saliva from25 male smokers...

Abstract There is a relationship between antioxidant activity and ageing stability of beer. The high-throughput capacity assays ORAC FRAP, which rely on specific reaction mechanisms, are validated as an addition to the established ESR-ST method with focus hop-derived antioxidants. Beers were brewed systematic variations in hop variety, product, hopping regime (late hopping) achieve sample beers...