Abstract Background The aims of the present study were to isolate and identify native entomopathogenic fungi, Beauveria bassiana from Egyptian soil evaluate artificial establishment B . as endophytes in rice plants through seeds immersion. Results Ten samples collected different cultivated fields at Ismailia Governorate. Only one sample was positive with a ratio 10%. Sequencing internal transcr...

The development of microtubule perturbing drugs is one the most promising anticancer therapeutic methods. Unfortunately, limitation such as drug resistance, adverse side effects, complex formulations and synthesis, limited bioavailability these has aroused search for a new molecule tubulin system. Different substituents chalcone were designed, synthesized, determined inhibition assembly toxicit...

Twelve novel β-lactams were synthesized and their antiproliferative effects and binding affinity for the predominant isoforms of the estrogen receptor (ER), ERα and ERβ, were determined. β-Lactams 23 and 26 had the strongest binding affinities for ERα (IC50 values: 40 and 8 nM, respectively) and ERβ (IC50 values: 19 and 15 nM). β-Lactam 26 was the most potent in antiproliferative assays using M...

Tubulin-binding cofactor A (TBCA) participates in microtubule formation, a key process in eukaryotic biology to create the cytoskeleton. There is little information on how TBCA might interact with β-tubulin en route to microtubule biogenesis. To address this, the protozoan Leishmania major was targeted as a model system. The crystal structure of TBCA and comparisons with three orthologous prote...

Between 2018 and 2021, several mastic trees (Pistacia lentiscus var. Chia) sampled in the field nursery of Chios Mastiha Growers Association (CMGA) were analyzed to determine cause dominant diseases. Symptoms included defoliation, leaf, twig blight, wilting and/or apoplexy young hardwood cuttings. Moreover, brown discoloration had also been observed on older woody parts such as branches tree tr...

BACKGROUND Phosducin-like protein 3 (PhLP3) forms a ternary complex with the ATP-dependent molecular chaperone CCT and its folding client tubulin. In vitro studies suggest PhLP3 plays an inhibitory role in β-tubulin folding while conversely in vivo genetic studies suggest PhLP3 is required for the correct folding of β-tubulin. We have a particular interest in the cytoskeleton, its chaperones an...

Microtubules are cytoskeletal filaments that are dynamically assembled from α/β-tubulin heterodimers. The primary sequence and structure of the tubulin proteins and, consequently, the properties and architecture of microtubules are highly conserved in eukaryotes. Despite this conservation, tubulin is subject to heterogeneity that is generated in two ways: by the expression of different tubulin ...

N-Phenyl-N'-(2-chloroethyl)ureas (CEUs) are antimicrotubule agents interacting covalently with β-tubulin near the colchicine-binding site (C-BS). Glutamyl 198 residue in β-tubulin (Glu198), which is adjacent to the C-BS behind the two potent nucleophilic residues, Cys239 and Cys354, has been shown to covalently react with 1-(2-chloroethyl)-3-(4-iodophenyl)urea (ICEU). By use of mass spectrometr...

Molecular approaches were used to re-evaluate the morphological criteria used to identify plant-associated Verticillium species. ITS-RFLPs divided the 31 studied strains of seven Verticillium species, (including the type species V. luteo-album) into four clusters. Cluster one comprised strains of the type species, V. luteo-album, cluster two V. albo-atrum, V. dahliae, V. nubilum and V. tricorpu...