Lipoic acid, an essential enzyme cofactor, is required in three domains of life. In the past 60 years since its discovery, most of the pathway for lipoic acid synthesis and metabolism has been elucidated. However, genetic control of lipoic acid synthesis remains unclear. Here, we report integrative evidence that bacterial cAMP-dependent signaling is linked to lipoic acid synthesis in Shewanella...

The use of gene fusion technologies for transcriptional analysis of gene expression, especially of those genes whose products are difficult to assay, has been well documented (12–15). Many narrow and broad-host-range plasmid-based systems are available for constructing such gene fusions (6,7, 14). To circumvent some of the inherent problems of plasmid-based systems, which have been discussed be...

Abstract Background Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates not introduced feedstocks oleaginous yeasts. Results In this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder c...

β-D-Galactosidase (EC 3.2.1.23) has been detected in several plant species, and is characterized in different organs and tissues by its ability to hydrolyse terminal non-reducing β-D-galactosyl residues from β-D-galactoside polymers. In the present paper the cloning and the biochemical and molecular characterization of Coffea arabica β-galactosidase expressed in the pericarp and the endosperm o...

β -D-Galactosidases (EC 3.2.1.23) hydrolyze the terminal nonreducing β -D-galactose residues in β -D-galactosides and are ubiquitously present in all life forms including extremophiles. Eighteen microbial β -galactosidase protein sequences, six each from psychrophilic, mesophilic, and thermophilic microbes, were analyzed. Primary structure reveals alanine, glycine, serine, and arginine to be hi...

since most of the studies on chemical compounds of dermatophytes have shown the existence of a relationship between their pathogenisity and proteolytec enzymes. activities of 19 different enzymes in viable mycelia and cytoplasmic extracts of t.rubrum (cetr), t.mentagrophytes (cetm) and t.verrucosum (cetv) were investigated by the api-zym system. the results showed that viable mycelia of t.rubrm...

Thermostable β-galactosidases from thermophilic bacteria have attracted increasing interest to various advantages in industrial and biotechnological applications. In this study, a highly thermally stable β-galactosidase produced by Anoxybacillus sp. AH1was purified characterized. The highest enzyme production was achieved after the bacterium incubated for 24 hours. precipitation with ammonium s...