Six diagnostic methods (Giemsa staining, Ziehl-Neelsen staining, auramine-rhodamine staining, Sheather's sugar flotation, an indirect immunofluorescence procedure, and a modified concentration-sugar flotation method) for the detection of Cryptosporidium spp. in stool specimens were compared on the following attributes: diagnostic yield, cost to perform each test, ease of handling, and ability t...

OBJECTIVES To determine the performance of the FASTPlaqueTB test, based on bacteriophage amplification technology, by comparison with the BACTEC 460 TB culture system, the Löwenstein-Jensen (LJ) medium culture method and Ziehl-Neelsen (ZN) staining. METHODS Of 400 sputum specimens studied in our laboratory, 19 were excluded due to contaminant growth. The FASTPlaqueTB test was performed accord...

Despite the recent development of more sensitive technologies, diagnosis of tuberculosis in resource limited settings continues to rely on sputum smear microscopy. This is explained by the fact that smear microscopy is simple, inexpensive, and the most accessible tool in resource limited settings for tuberculosis diagnosis. Hot Ziehl-Neelsen (Z-N) is still used in laboratories of resource limit...

UNLABELLED The resurgence of Mycobacterium tuberculosis in association with HIV infection has focussed much attention in the rapid diagnosis of high risk cases. Infection with HIV is known to alter the presentation of pulmonary tuberculosis. The present study was under taken to compare the efficacy of fluorochrome stain (Fl) with conventional Ziehl Neelsen (ZN) stain in the diagnosis of pulmona...

Background: Tuberculosis (TB) is a health problem worldwide. Cutaneous TB comprises a small fraction of all clinical forms of TB. Objective: The purpose of this study was to assess the disease pattern of cutaneous TB in an Asian country. Materials and Methods: Totally, 50 cases of cutaneous TB were diagnosed on histopathology in a tertiary care hospital over a period of 7 years. Relevant clinic...

Some techniques available at our laboratory were tested for their ability to aid in the morphological diagnosis of hydatid elements (Echinococcus granulosus ["Taenia echinococcus"]) isolated from cysts in humans and sheep. Unstained, methanol-fixed hooklets were fluorescent, most starkly so under violet light (excitation filter wavelength, 405 nm; long-pass filter wavelength, 495 nm). Auramine-...

background: cryptosporidium is a protozoan parasite causes cryptosporidial diarrhea , which is typically a short-lasting benign infection, but can become severe and non-resolving in immunocompromised individuals. the aim of the present study was to determinate the prevalence of cryptosporidium infection in livestock and humans that were in contact with livestock in hamadan district, iran. metho...

Objectives: Hansen’s disease is a chronic granulomatous infectious caused by Mycobacterium leprae. Early detection essential to prevent the disease’s spread and disability. Due multitude of morphologies, it may be difficult accurately diagnose certain cases based solely on clinical signs; therefore, histopathology crucial for definitive diagnosis. The study aims investigate various histological...

In this study, a case of pulmonary aspergillosis was described in 20-day-old suckling lamb. Lamb suffering from high fever, anorexia, weakness and balance disorder brought to the Department Pathology Kafkas University Faculty Veterinary Medicine for necropsy. According anamnesis obtained animal owner, an intense random drug treatment administered animal. Following systemic necropsy animal, vari...