Due to increasing numbers of patients with documented infections with Cryptosporidium and other coccidia, it is important for the physician and clinical laboratory to be aware of the appropriate diagnostic techniques necessary for organism recovery and identification. Although Cryptosporidium is found in the gastrointestinal tract, tissue biopsies may be insufficient for organism recovery; the ...

Some techniques available at our laboratory were tested for their ability to aid in the morphological diagnosis of hydatid elements (Echinococcus granulosus ["Taenia echinococcus"]) isolated from cysts in humans and sheep. Unstained, methanol-fixed hooklets were fluorescent, most starkly so under violet light (excitation filter wavelength, 405 nm; long-pass filter wavelength, 495 nm). Auramine-...

Method: Three hundred and twenty-nine patients were included in this study during the period from October 2004 to January 2006. The patients were examined for the presence of acid-fast bacilli. Thin bacterial smears were prepared and stained by Ziehl Neelsen (ZN) stain. Two tubes of the Lowenstein-Jensen (LJ) medium were inoculated with 20 μl of the neutralized sputum sample that was obtained f...

Sputum microscopy is the most important tool for detection of Mycobacterium tuberculosis in peripheral laboratories. Sodium hypochlorite concentration technique prior to sputum may improve AFB bacilli over direct Ziehl Neelsen’s staining method. A prospective study was conducted at Kasturba Medical College, Manipal. Consecutive samples were collected 3 months. ZN stained smears made directly fr...

ABSTRACT Mycobacteriosis was detected in seven out of one Atlantic mackerel (Scomber scombrus) that purchased for human consumption from a fish market. The apparently healthy but during cleaning, several granulomatous foci were noticed the visceral organs. Histopathological examination lesions revealed numerous characterized by caseous necrosis center lesion surrounded epithelioid giant cells. ...