Essential oils (EOs), which are derived from plants, have antifungal, insecticidal and antimicrobial activities, but they are slightly soluble in water and impart to the water their odor and taste, which limit their applications in food area. Zein, a prolamin from corn, is able to form nanoparticles by liquid-liquid dispersion process. These nanoparticles are well dispersed in water and stable,...

We investigated the effect of 3'-noncoding and poly(A) sequences on the translation and stability of membrane-bound mRNA (maize zein) and free mRNA (Xenopus beta-globin) by injecting SP6 transcripts into stage 6 Xenopus oocytes. With zein mRNA, the presence or absence of a 3'-noncoding or poly(A) sequence had little effect on mRNA stability over 24 h, and the 3'-noncoding sequence played essent...

Interactions of a model surfactant, sodium dodecyl sulfate (SDS), with a water-insoluble model protein, zein, were investigated to gain an understanding of the effects, such as skin irritation and protein denaturation, of surfactants that are common in personal-care products. To elucidate the mechanisms of such effects, the zeinprotein interactionwithSDSinaqueoussolutionswas investigatedusingam...

With the increasing awareness of environmental protection, interest in development biodegradable materials has become increasingly popular. At present, wheat gluten protein (WGP), soybean isolate (SPI), and Zein have made some progress application packaging materials. same time, beeswax (BW) is widely applied preparation food coatings. In this study, composite films were prepared by adding BW t...

Prolamins, the major cereal seed storage proteins, are sequestered and accumulated in the lumen of the endoplasmic reticulum (ER), and are directly assembled into protein bodies (PBs). The content and composition of prolamins are the key determinants for protein quality and texture-related traits of the grain. Concomitantly, the PB-inducing fusion system provides an efficient target to produce ...

The origin of protein bodies in maize (Zea mays L.) endosperm was investigated to determine whether they are formed as highly differentiated organelles or as protein deposits within the rough endoplasmic reticulum. Electron microscopy of developing maize endosperm cells showed that membranes surrounding protein bodies were continuous with rough endoplasmic reticulum membranes. Membranes of prot...

Opaque or nonvitreous phenotypes relate to the seed architecture of maize (Zea mays) and are linked to loci that control the accumulation and proper deposition of storage proteins, called zeins, into specialized organelles in the endosperm, called protein bodies. However, in the absence of null mutants of each type of zein (i.e. alpha, beta, gamma, and delta), the molecular contribution of thes...