berberine was loaded in yeast cells of saccharomyces cerevisiaeas a novel pharmaceutical carrier to improve the treatment ofmany diseases. the yeast-encapsulated active materialsshowedhigh stability and bioavailability due to the enhanced solubility and sustained releasing. in this study, different characteristics of prepared berberine loaded yeast cells (loading capacity, release kinetic order...

Ionic aluminum (Al3+) is toxic to plants, microbes, fish, and animals, but the mechanism of its toxicity is unknown. We describe the isolation of two yeast genes (ALR1 and ALR2) which confer increased tolerance to Al3+ and Ga3+ ions when overexpressed while increasing strain sensitivity to Zn2+, Mn2+, Ni2+, Cu2+, Ca2+, and La3+ ions. The Alr proteins are homologous to the Salmonella typhimurium...

Chaperones are produced by prokaryotic, yeast and higher eukaryotic cells for various purposes. Over-expression of each chaperone or sets of them affect the production level of a recombinant protein in the cell. On the basis of this hypothesis, five different plasmids with 5 different combinations of 6 chaperones molecule, transformed into Escherichia coli along with human basic Fibroblast Grow...

background : amelogenins are the major components of enamel matrix proteins. enamel matrix derivatives (emd) can be used in periodontal diseases to regenerate periodontal tissues. the main aim of this study was to evaluate ex-pression of full-length functional recombinant human amelogenin (rham) in iranian lizard leishmania (i.l.l.) as an alternative eukaryotic expression system. methods : huma...

Sequencing of the Caenorhabditis elegans genome revealed sequences encoding more than 1,000 G-protein coupled receptors, hundreds of which may respond to volatile organic ligands. To understand how the worm's simple olfactory system can sense its chemical environment there is a need to characterise a representative selection of these receptors but only very few receptors have been linked to a s...

Objective(s) To compare two identification methods, i.e., restriction fragment length polymorphism (RFLP)-PCR analysis and enzymatic method Rapid TM Yeast Plus System to identify different species causing vulvovaginal candidiasis (VVC). Materials and Methods Vaginal discharges of women who had attended the gynecology outpatient clinic of Mobini Hospital in Sabzevar, Iran were collected using...

background: the most important virulence factor which plays a central role in candida albicans pathogenesis is the ability of this yeast to alternate between unicellular yeast and filamentous hyphal forms. efg1 protein is thought to be the main positive regulating transcription factor, which is responsible for regulating hyphal-specific gene expression under most conditions. als3 is one of the ...

Abstract Vacuolar Phosphate Transporter1 (VPT1)-mediated phosphate uptake in the vacuoles is essential to plant development and fruit ripening. Interestingly, here we find that VPT1 may transport sugar response soluble status of fruits. The VvVPT1 protein isolated from grape (Vitis vinifera) berries was tonoplast-localized contains SPX (Syg1/Pho81/XPR1) MFS (major facilitator superfamily) domai...