The aim of this study was to efficiently use barley straw as a lignocellulosic feedstock for biobutanol production. Dilute sulfuric acid pretreatment was employed to solubilize hemicellulose in barley straw from cellulosic residues. The pretreated hydrolysate was cofermented with starch-based biomass in acetone–butanol–ethanol (ABE) fermentation. There were two co-fermentation processes: I) Bar...

A new fungus Cladosporium oxysporum GQ-3 producing extracellular xylanase was isolated from decaying agricultural waste and identified based on the morphology and comparison of internal transcribed spacer (ITS) rDNA gene sequence. C. oxysporum produced maximum xylanase activity of 55.92 U/mL with wheat bran as a substrate and NH4Cl as a nitrogen source. Mg(2+) improved C. oxysporum xylanase pro...

Although several xylanases have been studied, only few xylanases from marine micro-organisms have been reported. We report here a novel halotolerant xylanase from marine bacterium Bacillus subtilis cho40 isolated from Chorao island of mandovi estuary Goa, India. Extracellular xylanase was produced by using agricultural residue such as wheat bran as carbon source under solid-state fermentation (...

BACKGROUND Biofuels production from plant biomasses is a complex multi-step process with important economic burdens. Several biotechnological approaches have been pursued to reduce biofuels production costs. The aim of the present study was to explore the production in tobacco plastome of three genes encoding (hemi)cellulolytic enzymes from thermophilic and hyperthermophilic bacterium and Archa...

The most important building block of hemicelluloses is xylan. It is broken down into xylose oligomer residues by Xylanase an enzyme, produced by most organisms, to utilize xylose as primary source of carbon. The Xylanase produced are classified into families, viz 5, 8, 10, 11 and 43 of Glycoside Hydrolases (GH). Xylanase from family GH 11 are monospecific, they consist solely of Xylanase activi...

The xylanase gene from the ruminal bacterium Bacteroides ruminicola 23 is highly expressed in colonic Bacteroides species when carried on plasmid pVAL-RX. In order to stabilize xylanase expression in the absence of antibiotic selection, the xylanase gene was introduced into the chromosome of Bacteroides thetaiotaomicron 5482 by using suicide vector pVAL-7. Xylanase activity in the resulting str...

Hydrolysis and dehydration reactions of carbohydrates, which are used as energy raw materials by all living things in nature, controlled Carbohydrate Active Enzyme (CAZy) systems. These enzymes also different industrial areas today. There types microorganisms that have the CAZy system sector. Apart from current organisms, there rumen fungi within group candidate with system. It has been reporte...

Abstract In this study, the effect of pectin extraction method on properties cellulose nanofibers (CNFs) isolated from sugar beet pulp (SBP) was studied. Pectin extracted by industrially practiced sulfuric acid hydrolysis or enzymatic using a cellulase/xylanase enzymes mixture. The CNFs were then high-pressure homogenization and investigated in terms their chemical composition, crystallinity, s...