A growth medium with a specific oxidation-reduction potential containing peptone, dextrose, sodium succinate, sodium lactate, gelatin, sodium bicarbonate and blue tetrazolium, an indicator dye, in a tris(hydroxymethyl)aminomethane buffer was used to detect the presence of microorganisms in blood. The procedure involved the introduction of blood (and bacteria) into the growth medium with the dye...

The blood was collected as early as possible after onset of symptoms. In two patients the blood was collected 3 hours after onset, in four 4 hours, in four 5 hours, in five 7 hours and in five 9 hours. In twenty of the patients therefore the blood was collected within 9 hours of the onset of symptoms, in the remaining six after longer periods. Approximately 5 c.cm. 9 blood was withdrawn under t...

Blood culture-negative endocarditis (BCNE) may represent up to 70% of all endocarditis cases, depending on series. From 2001 to 2009, we implemented in our laboratory a multimodal diagnostic strategy for BCNE that included systematized testing of blood, and when available, valvular biopsy specimens using serological, broad range molecular, and histopathological assays. A causative microorganism...

The reliability of an in vitro pyrogen test system based on proinflammatory cytokine release from human monocytic cells was assessed by comparison with a test system based on a human whole blood culture as well as with the conventional rabbit pyrogen test. The human cells used as the pyrogen indicator cells were newly selected by subcloning of a human monocytic cell line, Mono-Mac-6. The select...

We present two experiments on the role of culture in the categorization of object part-whole structures. A triadic categorization task pitted shape against function as factors driving similarity judgments on selected parts of different types of objects. Speakers of American English were significantly more likely than speakers of two indigenous languages of Mexico, Tseltal Maya and Isthmus Zapot...