In this work, we report the effect of biopolymers (starch and gelatin) on in vitro biocompatibility property of nano hydroxyapatite (nHAp) composites. Cell culture and MTT assays were performed for in vitro biocompatibility. They show that nHAp can affect the proliferation of cells and the nHAp-starch and nHAP-gelatin biocomposites have no negative effect on the cell morphology, viability and p...

the gf677 (prunus amygdalus×prunus persica) is a peach rootstock tolerant to fe deficiency .therefore, mass propagation of gf677 rootstock is recommended by bioreactor. on the other hand, plant micro-propagation using bioreactors can decrease the cost of production and makes it an economical method. in this research, the possibility of using bioreactor and optimization of the tissue culture pro...

background: matrigel (extracellular matrix) can improve the growth of many cell types in vitro. objective: the aim of the present study was to determine the effect of matrigel on the development of 2-4 cells human embryos in culture. material and methods: surplus 2-4 cells human embryos, resulting from icsi, were divided into two groups (control and test). quality of embryos in both groups was ...

background: this study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (tcm199)–vero cells and menezo b2 (b2)-vero cells for the in vitro developmental quantity and quality of bovine embryos. materials and methods: bovine oocytes were allowed to mature and subsequently undergo fertilization in vitro. their presump...

In vitro cormogenesis is a potential tool for improving saffron production under controlled conditions. this study, the effects of explant type, culture and medium supplements on daughter corm formation in were assessed. Saffron flowers emerged 30 days after culture, sizes in-vitro- ex-vitro-produced stigmas similar. life cycle was completed 10 14 weeks respectively. Using intact corms more eff...

This review outlines and discusses the structure of liver, function liver cells, several methods tissue culture in vitro , applications .

Aloe plants can be grown autotrophically in a maintenance-free, sterile, ex vitro environment. Excel, 15-5-15 plus minors fertilizer formulation and Plant Preservative Medium (PPM) biocide was used as a very economical basal medium. In vitro micropropagated plantlets were regenerated in Excel media with 2 g /L sucrose and 2.2 μM 6-Benzylamino purine (BA) and used to initiate ex vitro cultures i...

This review offers a practically oriented introduction to follicle culture in vitro, focusing on mouse follicles, but with reference to other species. The main principles of follicle growth are addressed, including the constraints of tissue culture, methods of follicle isolation, and techniques for individual and collective culture of intact follicles. Culture systems that support a spherical o...

Few attempts at in vitro culture of mermithids have been undertaken. The various methods used to initiate cultures are described. The capacity of a range of media to promote growth and development of the nematodes has been evaluated and current approaches to in vitro outlined.