BACKGROUND Currently, the cryopreservation of embryos and oocytes is essential for assisted reproductive technology (ART) laboratories worldwide. This study aimed to evaluate the efficacy of the Kitasato Vitrification System (KVS) as a vitrification device for the cryopreservation of mouse embryos to determine whether this novel device can be adapted to the field of ART. METHODS In Experiment...

Whiteware compositions prepared using Pugu kaolin and industrial grade quartz and feldspar were studied with respect to varying sintering temperatures. The strength developed was correlated to mullite (3Al2O3.2SiO2) content and the microstructure. It was observed that within the vitrification temperature range, the strength mainly depended on the mullite content and the bulk density. Above the ...

objective: the aim of this study was to evaluate the effect of sucrose on follicular survival rate and the incidence of apoptosis in rat ovarian tissue following vitrification. methods: ovaries of approximately 5-week-old female wistar rats were divided randomly into three groups: control (non-vitrified), vi (ethylene glycol + dimethyl sulfoxide) and vii (eg + dmso + 0.25 mol/lit sucrose). vit...

_______________________________________________________________________________________ Abstract Vitrification may alter the cytoskeleton (microtubule, meiotic spindle, microfilament, etc.) and the subsequent fertilization rate of in vitro derived bovine oocytes. This study was conducted to evaluate the effect of vitrification and post-thawing incubation periods on the cytoskeleton and fertiliz...

The ability to cryopreserve human oocytes and store them indefinitely would be beneficial for cancer patients at risk of becoming sterile after therapy, allow women to delay reproduction, and alleviate religious concerns associated with embryo storage. In 1986, Chen was the first to report a pregnancy originating from a frozen–thawed human oocyte. Although over 100 babies have been born from oo...

OBJECTIVE Cryopreservation of immature testicular tissue should be considered as an important factor for fertility preservation in young boys with cancer. The objective of this study is to investigate whether immature testicular tissue of mice can be successfully cryopreserved using a simple vitrification procedure to maintain testicular cell viability, proliferation, and differentiation capaci...

BACKGROUND The Rapid-i is a new FDA cleared closed carrier for embryo vitrification. The cooling rate of - 1220°C/min is far lower than that reported with open vitrification systems such as the cryoloop (-15,000°C/min). Little published data is currently available on this device. This study presents our initial clinical data, as well as live birth outcomes, with the Rapid-i. The efficacy of thi...