Nitrate uptake by roots of cowpea (Vigna unguiculata) was measured using (15)NO(3) (-), and the energy cost to the root was estimated by respirometry. Roots of 8-day-old cowpea seedlings respired 0.6 to 0.8 milligram CO(2) per plant per hour for growth and maintenance. Adding 10 millimolar NO(3) (-) to the root medium increased respiration by 20 to 30% during the following 6 hours. This increas...

Superoxide dismutases are a family of metalloenzymes that catalyze the dismutation of superoxide radicals into molecular oxygen and hydrogen peroxide, and thus represent a primary line of defence against oxidative stress. The iron-containing superoxide dismutases are only found in prokaryotes and plants. The iron superoxide dismutase of Vigna unguiculata (cowpea) consists of two polypeptides of...

We report the detection of insulin-like antigens in a large range of species utilizing a modified ELISA plate assay and Western blotting. We tested the leaves or aerial parts of species of Rhodophyta (red alga), Bryophyta (mosses), Psilophyta (whisk ferns), Lycopodophyta (club mosses), Sphenopsida (horsetails), gymnosperms, and angiosperms, including monocots and dicots. We also studied species...

Although Cu is phytotoxic at Cu(2+) activities as low as 1-2 microM, the effect of Cu(2+) on the nodulation of legumes has received little attention. The effect of Cu(2+) on nodulation of cowpea (Vigna unguiculata (L.) Walp. cv. Caloona) was examined in a dilute solution culture system utilising a cation exchange resin to buffer solution Cu(2+). The nodulation process was more sensitive to incr...

A well saturated genomic map is a necessity for a breeding program based on marker assisted selection. To this end, we are developing genomic maps for cowpea (Vigna unguiculata 2N = 22) and mung bean (Vigna radiata 2N = 22) based on restriction fragment length polymorphism (RFLP) markers. Using these maps, we have located major quantitative trait loci (QTLs) for seed weight in both species. Two...

Symbiotic DNA sequences involved in nodulation by Rhizobium must include genes responsible for recognizing homologous hosts. We sought these genes by mobilizing the symbiotic plasmid of a broad host-range Rhizobium MPIK3030 (= NGR234) that can nodulate Glycine max, Psophocarpus tetragonolobus, Vigna unguiculata, etc., into two Nod- Rhizobium mutants as well as into Agrobacterium tumefaciens. Su...

El frijol caupí (Vigna unguiculata [L.] Walp.) es una leguminosa con alto valor nutricional y el conocimiento de su diversidad potencial agronómico contribuye a establecer las bases para conservación uso sostenible. Por lo anterior, objetivo este trabajo fue identificar características morfológicas comportamiento 20 cultivares en Yucatán. experimento se realizó campo, mediante un diseño bloques...

many powdery mildew fungi occur as anamorphic state on different host plants in guilan province, iran. the species have usually been identified as oidium spp. in order to identify teleomorphic species using anamorphic characteristics and host range, several infected plant specimens were collected from around guilan province during 2004-2011. based upon  the  results of the study 25 species were...