for identification of individuals and parentage control performed by cattle breeders in the czech republic, a novel finnish bovine genotypes™ panel 3.1was amplified by means of one multiplex polymerase chain reaction. bovine panel encompasses all the 12 str loci recommended by the international society for animal genetics (isag) for routine use in parentage testing and identification, including...

objective: bacillus cereus (b. cereus) is a gram-positive, spore-forming bacteria widely distributed in the environment. this bacteria is an opportunistic human pathogen that can cause diarrheal and emetic types of food poisoning. the diarrhea type of food poisoning can be caused by hemolysin bl (hbl), non-hemolytic (nhe), and cytotoxin k enterotoxins. rice is commonly contaminated with b. cere...

background and objectives:hbv and htlv-i are life threatening infectious agents in patients who receive blood and blood products. although serological methods have been proved to be useful, detection of these viruses has remained a challengingissue due to the many obstacles. by the advent of nucleic acid testing methods, especially in multiplex format, more precise detection is possible.the obj...

bovine brucellosis is a zoonotic disease distributed worldwide and characterized by abortion and reducedfertility in cows. since brucellosis eradication programme in iran uses vaccination, test, slaughter andquarantine as control measures, it is essential to distinguish vaccine strains from strains that cause infectionsamong vaccinated cattle herds. we developed and evaluated a multiplex polyme...

Existing reactions for the multiplex PCR amplification of exons in the dystrophin gene have been modified to produce two multiplex reactions which separately cover the 5' and 3' major deletion 'hotspots' in the gene, and together detect approximately 98% of all deletions detectable by Southern cDNA hybridisation. A comparative study of 148 patients showed mistypings in both the cDNA hybridisati...

For the diagnosis of unexplained male infertility a multiplex PCR for 6 markers, which are well-known as candidate genes for studying male infertility and located on the human Y-chromosome, has been designed. The multiplex PCR products have been separated on a 12 channel microchip electrophoresis system, which can analyze different samples simultaneously. By combining the technologies of multip...

Contamination of powdered infant formula (PIF) by the bacteria Cronobacter spp. and Salmonella enterica was deemed a matter of great concern by the World Health Organization and the Food and Agriculture Organization of the United Nations in 2004. Therefore, we developed a rapid and sensitive multiplex real-time PCR assay for the simultaneous detection of Cronobacter and Salmonella in PIF. In ad...

OBJECTIVE To develop a technique for simultaneous detection of various target genes in Roundup Ready soybean by combining multiplex PCR and low-density DNA microarray. METHODS Two sets of the multiplex PCR system were used to amplify the target genes in genetically modified (GM) soybean. Seventeen capture probes (PCR products) and 17 pairs of corresponding primers were designed according to t...