Total protein synthesis in perfused livers of fed rats was determined by measuring the rate of valine incorporation based on the specific activity of valine attached to tRNA. Rates were not significantly altered when perfusate valine was increased from 0.40 to 5 mM and were similar to values calculated earlier from the specific activity of extracellular valine at a concentration of 15 mM. Overa...

The subcellular distribution of free amino acids was examined in 0.25 M sucrose homogenates of rat livers cycbtally perfused for 60 min with an unsupplemented (control) medium. An unexpectedly high (30%) proportion of total intracellular valine was found in the mitochondrial-lysosoma1 fraction; 57% was in the 100,000 x .o supernatant; and 9% and 4% were in the nuclear and microsomal fractions, ...

An in vitro protein-synthesizing system completely dependent on added valine tRNA (valyl-tRNAval) and programmed with RNA from the phage MS2 has been used to investigate the incorporation into MS2 coat protein of valine from isoaccepting valyl-tRNAsval with the anticodons U AC (U represents 5-oxyacetic acid uridine monophosphate), GAC, and IAC in response to the four valine codons GUU, GUC, GUA...

Incorporation of selected amino acids by resting cells was studied with regard to effects of concentration, rate and extent of incorporation, cellular distribution, effect of ammonium ion on uptake, and competitive effects. l-Aspartic acid, l-alanine, l-serine, and glycine presented at trace levels were incorporated at rates ranging from 0.11 to 8.2 mumumoles per mg (dry weight) per minute, and...

People's sensitivity to reinforcing stimuli such as monetary gains and losses shows a wide interindividual variation that might in part be determined by genetic differences. Because of the established role of the dopaminergic system in the neural encoding of rewards and negative events, we investigated young healthy volunteers being homozygous for either the Valine or Methionine variant of the ...

Chlamydiaceae are obligate intracellular bacterial pathogens that strictly depend on host metabolites, such as nucleotides, lipids, and amino acids. Depletion of amino acids in cell culture media results in abnormal chlamydial development in vitro. Surprisingly, enrichment of certain amino acids also retards chlamydial growth. Our experiments revealed that the antichlamydial effects are largely...

The subcellular distribution of free amino acids was examined in 0.25 M sucrose homogenates of rat livers cycbtally perfused for 60 min with an unsupplemented (control) medium. An unexpectedly high (30%) proportion of total intracellular valine was found in the mitochondrial-lysosoma1 fraction; 57% was in the 100,000 x .o supernatant; and 9% and 4% were in the nuclear and microsomal fractions, ...

Wang Ch., Xu F., Li D., Zhang M. (2015): Physico-chemical and structural properties of four rice bran protein fractions based on the multiple solvent extraction method. Czech J. Food Sci., 33: 283–291. The physicochemical and structural properties of the concentrated rice bran protein (CRBP) and rice bran protein fractions – RBPF (albumin, globulin, prolamin, and glutelin) were investigated on ...