A carcinoembryonic antigen (CEA)-producing human lung cancer cell line (A549), a nonproducing human lung cancer cell line (CADO-LC9), and a human uterine cervical cancer (HeLa) were transfected with the herpes simplex virus thymidine kinase (HSV-TK) gene regulated by 445 nucleotides upstream from the translational start of CEA gene. Fifty % growth inhibitory concentration of ganciclovir (GCV) w...

BACKGROUND Herpes simplex keratitis (HSK) caused by herpes simplex virus 1 (HSV-1), which has high recurrent rate and incidence of severe vision loss, is the leading cause of infectious blindness in the world. The aim was to explore the clinical efficacy of oral ganciclovir (GCV) in the prevention of recurrent HSK. METHODS A multicenter, prospective, randomized, single-blind, and controlled c...

Untuk mencapai terciptanya kehidupan bermasyarakat yang aman dan damai, tindak kriminal menjadi salah satu hal sangat diperhatikan. Pada tahun 2020, di Indonesia terjadi 6.872 kejadian kejahatan terhadap kesusilaan 36.672 fisik. Salah upaya bisa dilakukan untuk menekan jumlah fisik adalah dengan memodelkan tersebut atas faktor-faktor memengaruhinya sehingga dapat diperoleh prediksinya. peneliti...

For practical engineering structures, it is usually difficult to measure external load distribution in a direct manner, which makes inverse identification important. Specifically, typical problem, for the models (e.g., response matrix) are often ill-posed, resulting degraded accuracy and impaired noise immunity of identification. This study aims at identifying loads stiffened plate structure, t...

The topic of my dissertation research is Bayesian methods in the social sciences. There will be several parts to the dissertation. One part, the focus of this proposal, is a novel method called tuned Bayesian model averaging (tBMA) that modifies Bayesian model averaging (BMA) by using the data to inform the prior specification. BMA is a tool that accounts for model uncertainty in typical regres...

COVID-19 sangat mempengaruhi segala aspek kehidupan dalam masyarakat salah satunya tidak hanya perlambatannya bidang ekonomi, kesehatan, namun di pendidikan pun terkena dampak besar akibat dari ini. Dilihat indikator biasanya seseorang yang memiliki tinggi peluang diterima pada suatu perusahaan akan semakin besar. Semakin tingkat maka capaian rata-rata lama sekolah pula. Maka itu penelitian ini...

Suicide gene therapy systems such as the herpes simplex thymidine kinase/ganciclovir system (TK/GCV) may kill cancer cells by apoptosis through as yet undefined mechanisms. Here we show that TK/GCV treatment induces p53 accumulation and increases cell surface expression of CD95 and tumor necrosis factor receptor, which is likely to involve p53-mediated translocation of CD95 to the cell surface....

Acyclovir (ACV) has shown efficacy in the prophylactic suppression of human cytomegalovirus (HCMV) reactivation in immunocompromised renal transplant patients without the toxicity associated with ganciclovir (GCV). The HCMV UL97 gene product, a protein kinase, is responsible for the phosphorylation of GCV in HCMV-infected cells. This report provides evidence for the phosphorylation of ACV by UL...

Cytomegalovirus (CMV) replicates rapidly in its human host with a doubling time of approximately 1 day. Using simple mathematical models we show that the efficacy of the anti-CMV drug ganciclovir (GCV) against wild-type strains is 91.5% [95% confidence interval (CI) 89-94%] when given i.v. (5 mg/kg twice a day) but only 46.5% (95% CI 45-47.5%) when given orally (1 g three times a day) whereas t...

Thirteen point mutations targeting predicted domains conserved in homologous protein kinases were introduced into the UL97 coding region of the human cytomegalovirus. All mutagenized proteins were expressed in cells infected with recombinant vaccinia viruses (rVV). Several mutations drastically reduced ganciclovir (GCV) phosphorylation. Mutations at amino acids G340, A442, L446, and F523 result...