in this study, caffeic acid (ca) and its three derivatives including 3-caffeoylquinic acid (3-cqa, neochlorogenic acid), 4-caffeoylquinic acid (4-cqa, cryptochlorogenic acid), and 5-caffeoylquinic acid (5-cqa, chlorogenic acid) were identified in bupleurum chinense aerial parts using reverse-phase high-performance liquid chromatography (rp-hplc) with photodiode array (pda) detector, reference c...

The polyhedral building blocks of the layered inorganic network in the mixed-valence title compound, (NH4)(V(IV)O2)(V(V)O2)(TeO3), are vertex-sharing V(V)O4 tetra-hedra, distorted V(IV)O6 octa-hedra and TeO3 pyramids, which are linked by V-O-V and V-O-Te bonds, forming double layers lying parallel to (100). The presumed Te(IV) lone-pairs of electrons appear to be directed inwards into cavities ...

BACKGROUND PCR-based mutation detection is prone to methodological errors, e.g., in restriction length fragment polymorphism (RFLP) and allele-specific amplification (ASA), false PCR results may occur because of technical faults or atypical new mutations. METHODS We investigated the ability of a genotyping assay based on hybridization of labeled oligonucleotides to detect and discriminate kno...

The action of species cytoplasm specific (scs) gene(s) can be observed when a durum (Triticum turgidum L.) nucleus is placed in the Aegilops longissimum S. & M. cytoplasm. This alloplasmic combination, (lo) durum, results in nonviable progeny. A scs gene derived from T. timopheevii Zhuk. (scs(ti)) produced compatibility with the (lo) cytoplasm. The resulting hemizygous (lo) scs(ti)- durum line ...

OBJECTIVE To amplify the description of myocardial stunning. DESIGN Control versus 30 min after a 20 min no flow ischaemia. EXPERIMENTAL ANIMALS 15 isolated rabbit hearts perfused with erythrocyte suspension. MAIN OUTCOME MEASURES Left ventricular systolic function in terms of aortic flow, peak systolic pressure (LVPmax), dP/dtmax, and the end systolic pressure-volume relation (ESPVR); ea...

1. The diversity of alpha(2) and purinergic autoreceptor actions on action potential evoked calcium transients in single varicosities has been investigated using the calcium indicator Oregon Green 488 BAPTA-1. 2. During long trains of impulses (10 Hz for 30 s), the change in calcium concentration in varicosities from its resting level (Delta[Ca(2+)](v)) increased in many varicosities during the...

The anticancer drug cis-diammindichloroplatin (CDDP, cisplatin) causes severe side effects like peripheral sensitive neuropathy. The toxicity of CDDP has been linked to changes in intracellular calcium homeostasis ([Ca2+]i). Voltage activated calcium channel currents (ICa(V)) are important for the regulation of [Ca2+]i; therefore, this study was designed to examine the effect of CDDP on ICa(V) ...

Hydrosol is a side product of essential oil extraction from plants. In this study, the effect of various ratios (5-100 %v/v) of cinnamon hydrosol replacement in the formulation of edible gelatin films was investigated. Physical properties were evaluated and the antibacterial properties of gelatin films against Staphylococcus aureus, Staphylococcus saprophyticus and Staphyl...

A tetraplex PCR method was developed for simultaneous detection of Vibrio cholerae, V. parahaemolyticus, V. vulnificus and V. mimicus in cockle samples in comparison with conventional culture method. Specific primers targeting ompW of V. cholerae, tl of V. parahaemolyticus, hsp60 of V. vulnificus and sodB of V. mimicus were employed in the same PCR. Detection limit of the tetraplex PCR assay wa...