having knowledge about genetic relationships among accessions is necessary for developing breeding strategies to produce improved cultivars. in present study, genetic diversity and inter-relationship among 29 genotypes of citrus were comparatively analyzed using morphological and rapd markers. significant variability was observed among citrus genotypes for 61 quantitative and qualitative morpho...

Using PEG-mediated protoplast fusion, a total of nine pfle somatic hybrids were developed between Pleurotus florida and Lentinula edodes. Only six could fruit bodies the second generation was made each through tissue culture isolate from their bodies. The first denoted as pfle1p, pfle1q, pfle1v, 1s, pfle1o pfle1pFB, pfle1qFB, pfle1vFB, 1sFB, pfle1oFB. genetic closeness, stability variance is an...

Random amplified polymorphic DNA (RAPD) analysis was used to evaluate genetic diversity among 13 soil Penicillium strains originating from widely dispersed areas. Twenty one of the 34 synthetic random primers were found to identify polymorphism in amplification products. The results show a high level of diversity of RAPD markers among the strains. All the strains could be identified by their ch...

The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served...

Molecular and morphometric analyses of different Indian and Sri Lankan populations of two rattan species (Calamus rivalis and C. metzianus) were carried out using Random Amplified Polymorphic DNA (RAPD) markers and morphological characters. Multivariate analysis of RAPD and morphological data failed to separate these populations into two distinct species. RAPDs generated a total of 117 markers ...

abstract soybean (glycine max) is an important crop plant which contains a high amount of oil and protein. to evaluate the genetic diversity of 45 soybean genotypes growing in iran, two types of molecular markers (rapd and isj) were used. two sets of different primers were used to compare potential differentiation power of it (intron targeting) and et (exon targeting) primers. data obtained fro...

Abstract Fusarium head blight (FHB) is consistently one of the most important barley diseases worldwide. This study aimed to evaluate pathogenicity 16 isolates four species under controlled conditions and their genetic variability using 22 random amplified polymorphic DNA (RAPD) markers. Pathogenic variation was characterized based on disease development rates index two Syrian landraces with va...

background: as for human trichomoniasis the host-parasite relationship is very complex, and the broad ranges of clinical symptoms are unlikely be attributable to a single pathogenic mechanism. specific random amplified polymorphic dna (rapd) markers of 490 bp, 720 bp and 460 bp using the primers tv-5, opa-6 and opa-11, respectively, were reported. this was the first description of possible ge­n...

Propagation through tissue culture by using orchid seed as explants will produce a lot of plants. This study aims to measure the genetic character plantlets that were regenerated from seeds which have been resulted in vitro culture. The original plants produced was determined Random Amplified Polymorphic DNA (RAPD) molecular markers. results showed primers used RAPD analysis polymorphic band pa...

The development of DNA markers that can closely discriminate between Liriope and Ophiopogon species is vital for efficient and accurate identification of these species, and to ensure the quality, safety, and efficacy of medicines made from these plants. We developed species-specific molecular markers for these two genera. Forty RAPD primers were tested to detect polymorphism; species-speci...