The gold standard for the diagnosis of Helicobacter pylori infection requires an endoscopic biopsy of gastric mucosa for histological examination, urease test and culture. Noninvasive serological tests are useful as a screening test for H. pylori infection. The aim of this study was to evaluate the performance of a rapid office-based serologic test, using immunochromatography ICM, and the immun...

Objective: To investigate the presence of Helicobacter pylori in sinonasal mucosa of patients with chronic sinusitis Design: A prospective case-control study Materials and methods: Mucosal specimens were collected from the mid-third middle meatus and lateral side of mid-cornea. H. pylori has been investi...

METHODS: Seventeen patients diagnosed with central serous chorioretinopathy were examined for gastric infection with Helicobacter pylori using the urease test and gastric biopsy. Helicobacter pylori-positive patients were treated with the appropriate medication. The response to therapy was monitored by evaluating the best-corrected visual acuity and optical coherence tomography. The data were a...

The nickel-containing enzyme urease is an essential colonization factor of the gastric pathogen Helicobacter pylori, as it allows the bacterium to survive the acidic conditions in the gastric mucosa. Although urease can represents up to 10% of the total protein content of H. pylori, expression of urease genes is thought to be constitutive. Here it is demonstrated that H. pylori regulates the ex...

The effect of Indigenous microorganisms (IMO’s) on the native soil was investigated in the present study. Supplementation of IMO’s suspension to the soil alters the physico-chemical, biological and enzyme properties of the soil. These alternations include decreases in P from 7.2 to 6.8, increase in electrical conductivity 0.36 to 1.21 (μmohs/cm), water holding capacity 0.36 to 2.2ml/g of soil o...

Studies with two uropathogenic urease-producing Escherichia coli strains, 1021 and 1440, indicated that the urease genes of each are distinct. Recombinant plasmids encoding urease activity from E. coli 1021 and 1440 differed in their restriction endonuclease cleavage sites and showed minimal DNA hybridization under stringent conditions. The polypeptides encoded by the DNA fragments containing t...

Urease is a nickel-containing urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism in plants. The process of urease activation by incorporation of nickel into the active site is a prime example of chaperone-mediated metal transfer to an enzyme. Four urease accessory proteins are required for activation in Klebsiella aerogenes. In plants urease accessory pro...

Urease was purified 500-fold to electrophoretic homogeneity from ground, dry soybeans. Sodium dodecyl sulfate-acrylamide gel electrophoresis indicates a subunit size of 93,500 daltons which is identical with that of jack bean urease. In solutions of high ionic strength, there exists a single urease species (species 1) with a size of about 480,000 daltons based on agarose column chromatography a...

Proteus mirabilis urease, a nickel-containing enzyme, has been established as a critical virulence determinant in urinary tract infection. An amino acid sequence (residues 308 to 327: TVDEHLDMLMVCHHLDPSIP) within the large urease subunit, UreC, is highly conserved for every urease examined thus far and has been suggested to reside within the enzyme active site. Histidine residues have been post...