نتایج جستجو برای: urea transporter b expression

تعداد نتایج: 1715338  

Bacillus clausii TnrA transcription factor is required for global nitrogen regulation. In order to obtain anoverview of gene regulation by TnrA in B. clausii KSMK16, the entire genome of B. clausii was screened forthe consensus sequence, 5’-TGTNAN7TNACA-3’ known as the TnrA box, and 13 transcription units werefound containing a putative TnrA box. The TnrA targets identified in...

2010
Guangping Chen Yuan Yang Otto Fröhlich Janet D. Klein Jeff M. Sands

Chen G, Yang Y, Fröhlich O, Klein JD, Sands JM. Suppression subtractive hybridization analysis of low-protein dietand vitamin D-induced gene expression from rat kidney inner medullary base. Physiol Genomics 41: 203–211, 2010. First published March 2, 2010; doi:10.1152/physiolgenomics.00129.2009.—Protein restriction and hypercalcemia result in a urinary concentrating defect in rats and humans. P...

Journal: :American journal of physiology. Regulatory, integrative and comparative physiology 2009
P Tickle A Thistlethwaite C P Smith G S Stewart

Our previous studies have detailed a novel facilitative UT-B urea transporter isoform, bUT-B2. Despite the existence of mouse and human orthologs, the functional characteristics of UT-B2 remain undefined. In this report, we produced a stable MDCK cell line that expressed bUT-B2 protein and investigated the transepithelial urea flux across cultured cell monolayers. We observed a large basal urea...

2016
Marko Gröger Knut Rennert Benjamin Giszas Elisabeth Weiß Julia Dinger Harald Funke Michael Kiehntopf Frank T. Peters Amelie Lupp Michael Bauer Ralf A. Claus Otmar Huber Alexander S. Mosig

Liver dysfunction is an early event in sepsis-related multi-organ failure. We here report the establishment and characterization of a microfluidically supported in vitro organoid model of the human liver sinusoid. The liver organoid is composed of vascular and hepatocyte cell layers integrating non-parenchymal cells closely reflecting tissue architecture and enables physiological cross-communic...

Journal: :The Biochemical journal 2011
Robert J Vandenberg Cheryl A Handford Ewan M Campbell Renae M Ryan Andrea J Yool

Glutamate transport is coupled to the co-transport of 3 Na(+) and 1 H(+) followed by the counter-transport of 1 K(+). In addition, glutamate and Na(+) binding to glutamate transporters generates an uncoupled anion conductance. The human glial glutamate transporter EAAT1 (excitatory amino acid transporter 1) also allows significant passive and active water transport, which suggests that water pe...

Journal: :Circulation Research 2001

Journal: :The Journal of experimental biology 2013
Jesper Brahm

This study extends permeability (P) data on chloride, urea and water in red blood cells (RBC), and concludes that the urea transporter (UT-B) does not transport water. P of chick, duck, Amphiuma means, dog and human RBC to (36)Cl(-), (14)C-urea and (3)H2O was determined under self-exchange conditions. At 25°C and pH 7.2-7.5, PCl is 0.94 × 10(-4)-2.15 × 10(-4) cm s(-1) for all RBC species at [Cl...

Journal: :American journal of physiology. Renal physiology 2006
Gavin S Stewart Craig P Smith Gordon J Cooper

The amphibian urea transporter (fUT) shares many properties with the mammalian urea transporters (UT) derived from UT-A and UT-B genes. The transport of urea by fUT is inhibited by the mercurial agent p-chloromercuribenzenesulfonic acid (pCMBS). We found that in oocytes expressing cRNA encoding fUT, a 5-min preincubation in 0.5 mM mercury chloride (HgCl2) also significantly reduced urea uptake....

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