Acute kidney injury (AKI) has a high incidence on intensive care units around the world and is a major complication in critically ill patients suffering from sepsis or septic shock. The short- and long-term complications are thereby devastating and impair the quality of life. Especially in terms of AKI staging, the determination of kidney function and the timing of dialytic AKI management outsi...

This reduces the number of reagents required for color development to two, each of which is stable for 60 days or more if kept cool and in amber bottles protected from light. These reagents are added in equal volumes to the ammonia sample. For example, to 1-5 g. of ammonia in a volume of 1 ml. or less, 5 ml. each of diluted solutions 1 and 2 are added successively. Maximum blue color is produce...

Ultra-fast liquid chromatography with a photodiode array detector for simple and rapid determination of urea in body fluids of farm animals is described. Blood plasma, milk, and urine samples are treated with trichloroacetic acid and then centrifuged. Supernatants are derivatized at room temperature using p-dimethylaminobenzaldehyde. Samples are separated using a ternary gradient of methanol in...

chemistry analyzer, is intended to be used for the in vitro quantitative determination of alanine aminotransferase (ALT), albumin, alkaline phosphatase (ALP), aspartate aminotransferase (AST), calcium, chloride, creatinine, glucose, potassium, sodium, total bilirubin, total carbon dioxide, total protein, and blood urea nitrogen (BUN) in heparinized whole blood, heparinized plasma, or serum in a...

To investigate the pharmacokinetics and pharmacodynamics of Astragali Radix-Corni Fructus herb-pair in kidney-yin deficiency model, which was made by continuously oral gavage thyroxine. A simple rapid LC-MS/MS method developed validated for determination loganin morroniside rat plasma used study. The significantly changed AUC(0-∞), Cmax CLz/F morroniside. T1/2z increased rats. For study, liver ...

THE possibility of an error in the determination of urea in blood by urease methods was brought to our notice by the fact that figures obtained by the direct estimation of urea in human blood corpuscles were obviously too high. This error appears to have been first reported by Behre [1923], who observed that when the soya bean urease method was applied to whole blood the urea obtained was parti...

The objective of the present work was to compare colorimetric and distillationÈtitration methods to determine the ammonia-nitrogen con3 centration in sheep and goat urine samples. Colorimetric methods used were based on the indophenol reaction, whereas titrimetric methods were based on the alkali distillation of ammonia from the urine and its titrimetric determination with a standard acid. Colo...

Quantitative spectrophotometric analysis of poorly water-soluble drugs involves use of various organic solvents. Major drawbacks of organic solvents include high cost, volatility and toxicity. Safety of analyzer is affected by toxicity of the solvent used. In the present investigation the use of organic solvent has been avoided, making the method environmentally friendly. Urea has demonstrated ...

AIMS To optimise the ELISA method for the avidity of IgG antibodies against neurofilament heavy chain (NfH) and to determine the levels and avidity of anti-NfH antibodies in patients with Alzheimer's disease (AD) and a healthy control group. METHODS Various dilutions of sera and concentrations of urea and sodium chloride as chaotropic reagents were tested in the process of the ELISA optimisat...

An automatic enzyme kinetic luminometric method for determination of small quantities of urea in biological fluids and in microdialysates is presented. The method is based on the ATP-hydrolyzing urease reaction [urea amidohydrolase (ATP-hydrolyzing); EC 3.5.1.45], monitored by a luciferin-luciferase ATP reaction. The assay range is 100 pmol to 50 nmol with a detection limit of 5 micromol/L in t...