Most of our current knowledge of cellular ultrastructure is derived from studies of chemically fixed and chemically cryoprotected preparations. In the first part of this review, we document the many artifacts associated with chemical techniques that render them unsuitable for further refinement of our understanding of cellular ultrastructure. The best method currently available for the preserva...

Purpose: To investigate the effects of dexmedetomidine (DEX) on amino acid contents and the cerebral ultrastructure of rats with cerebral ischemia-reperfusion injury (I/R). Methods: Thirty-six, male, Wistar rats were randomly divided into three groups: the sham operation group (group C), the ischemia-reperfusion group (group I/R), and the DEX group (group D). The middle cerebral artery occl...

Our previous studies have shown that licochalcone A, an oxygenated chalcone, has antileishmanial (M. Chen, S.B. Christensen, J. Blom, E. Lemmich, L. Nadelmann, K. Fich, T.G. Theander, and A. Kharazmi, Antimicrob, Agents Chemother. 37:2550-2556, 1993; M. Chen, S.B. Christensen, T.G. Theander, and A. Khrazmi, Antimicrob. Agents Chemother. 38:1339-1344, 1994) and antimalarial (M. Chen, T.G. Theand...

Viral pathogens are major causes of outbreaks in shrimp farms throughout the world. Monodon baculovirus has been known to be invasive in 85-100% of the shrimp hatcheries, in early or late stages of shrimp. Three-hundred and sixty juvenile of Litopenaeus vannamei with average (±SD) size of 7.99±0.54 g and 3600 post larvae 10-15 were prepared from Shrimp Research Station located in Helleh and 3 h...

reported that the nodule in Still's disease often resembled that of rheumatic fever histologically; this was based on biopsies of eleven cases compared with 57 with rheumatic fever and 22 with adult rheumatoid arthritis. We now report a small series of six children followed for 3 to 5 years, where multiple small nodules as seen clinically in rheumatic fever were the predominant clinical feature...

Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not reported previously: intine fibrils, intine vesicles, intine membrane, and mi...

J. Appl. Phys. 30: 2038, 1959.12. FERN.A_NDEZ-MORkN, H.: Multiple objective aper-tures for high-resolution electron mnicroscopy.Abstract. J.Appl. Phys. 30: 2038, 1959.18:). FERN\.&NDEZ-MORaN;, 11.:Low-Temperature preparationi techniques for electroni microscopy of biological specimens based on rapid freezing w ith heliuni II . In Conference onFreezinglaind IDrv-illg o...

J. Appl. Phys. 30: 2038, 1959.12. FERN.A_NDEZ-MORkN, H.: Multiple objective aper-tures for high-resolution electron mnicroscopy.Abstract. J.Appl. Phys. 30: 2038, 1959.18:). FERN\.&NDEZ-MORaN;, 11.:Low-Temperature preparationi techniques for electroni microscopy of biological specimens based on rapid freezing w ith heliuni II . In Conference onFreezinglaind IDrv-illg o...

Sarcoma 180 has been studied with the electron micro scope. Desmosomes have been demonstrated at the intercel lular junctions in the tumor cells. The desmosome consists of a plate-like cytoplasmic condensation on which bundles of tonofilaments end. The intercellular space at the desmo some level may show a dense, fibrillar material. The presence of desmosomes and tonofilaments demonstrates the ...

Many fungi have been identified as pathogens of marine algae. Among them, Chytridiomycota revealed relatively highly abundant, but much the diversity known within these groups is almost entirely based on environmental sequencing data. Here, we present a novel chytridiomycete genus and species, characterized by light microscopical observations, ultrastructure, molecular phylogenetic analysis par...